Laboratory Diagnosis

Objective. To assess efficacy of PCR for detection of Legionella in bronchoalveolar lavage (BAL) fluid obtained from hematological patients with pneumonia and acute respiratory failure. Materials and Methods. A total of 79 BAL samples collected from the hospitalized hematological patients with pneumonia and acute respiratory failure (during the 2011–2014) were analyzed using PCR. All BAL samples have been tested for legionellosis by culture immediately after collection, and then stored at –70 °C.

The objective of this study was to develop algorithm of microbiological diagnosis for chronic lung infection in patients with cystic fibrosis and implement it for monitoring of chronic infection in children and adults. Data from our own studies …

Objective. To compare results of species identification of yeast isolated from vaginal discharge in women with vulvovaginal candidiasis using conventional microbiological methods and MALDI-TOF MS. Materials and Methods. A total of 2304 isolates of yeast were tested by MALDI-TOF MS method. Species identification of 1294 isolates was also performed using conventional methods. Results. According to score values (indicates accuracy of identification), the best results (score 2-2.3) were yielded in 55.

Objective. To assess the use of MALDI-TOF MS and the experimental «Septi-Panel» assay (quantitative PCR method) in the screening of pathogens in blood culture. Materials and Methods. A total of 50 positive blood cultures (BactAlert) obtained from adult patients and newborns with suspected sepsis were included in the study. Pathogen detection were performed by direct mass-spectrometry, quantitative PCR using «Septi-Panel» assay, and culture. Results. Of 50 positive blood cultures tested, 76% and 92% of microorganisms were identified to species by direct mass-spectrometry and quantitative PCR, respectively.

The review deals with modern methods of indication of infectious agents in the blood. From methodological point of view, they can be divided into two main groups: bacteriologic and molecular. The first group of methods used and improved almost from …

Laboratory diagnostics are performed for variety of biological materials and are pursuing a purpose of objective evaluation of variable biological objects. Therefore the selection and provision of stabile, verified conditions for obtaining …

Over the last decades there is an uncontrollable rise of MRSA prevalence. Infections caused by MRSA are associated with high mortality and healthcare costs. New antimicrobial drugs (anti-MRSA cephems, new glycopeptides, lipopeptides, glycilcyclines …

Results of the comparative susceptibility testing of Gram-negative aerobic bacteria by disc diffusion method on Mueller-Hinton Agar (MHA) and the AGV medium that are widely used for susceptibility testing in Russia, are presented. The aim of the …

These guidelines present the general requirements to biological specimens assigned for microbiological testing. Instructions on specimen collection in the management of different infections are described. Recommendations on interpretation of …

The approach to administration of antimicrobials is often based on the results of microbiological studies that include microorganism isolation, identification and, most important, susceptibility testing. Majority of microbiological laboratories in …