MRSA Typing: Which Methods are the Most Appropriate for Different Purposes?

Clinical Microbiology and Antimicrobial Chemotherapy. 2011; 13(2):168-176

Journal article


Over the last decades there is an uncontrollable rise of MRSA prevalence. Infections caused by MRSA are associated with high mortality and healthcare costs. New antimicrobial drugs (anti-MRSA cephems, new glycopeptides, lipopeptides, glycilcyclines and others) are only introducing into clinical practice. Given this, control of MRSA distribution is of great importance. Currently, molecular typing methods, which replace phenotypic methods of microorganism comparison, are widely used for these purposes. This paper provides the data on the most common typing methods of S. aureus and scope of their use. In our point of view, use of pulsed field gelelectrophoresis (PFGE) or multilocus VNTR analysis in combination with SCCmec typing is the most appropriate for studying local MRSA epidemiology. In order to investigate global MRSA epidemiology and population structure, methods such as multilocus sequence typing (MLST) and spa typing, may be recommended. These methods provide a high level of reproducibility and possibility for exchanging data between different laboratories all over the world.

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