Comparative Assessment of Species Identification of Vaginal Yeast Isolates Using MALDI-TOF MS and Conventional (Biochemical and Phenotypic) Methods

Clinical Microbiology and Antimicrobial Chemotherapy. 2014; 16(1):10-17

​ Muravyova V.V., Priputnevich T.V., Zavyalova M.G., Ankirskaya A.S., Ilyina E.N.
vulvovaginal candidiasis yeast massspectrometry MALDI-TOF MS
Section
Laboratory Diagnosis
Type
Journal article
Publication
Clinical Microbiology and Antimicrobial Chemotherapy. 2014; 16(1):10-17

Objective.

To compare results of species identification of yeast isolated from vaginal discharge in women with vulvovaginal candidiasis using conventional microbiological methods and MALDI-TOF MS.

Materials and Methods.

A total of 2304 isolates of yeast were tested by MALDI-TOF MS method. Species identification of 1294 isolates was also performed using conventional methods.

Results.

According to score values (indicates accuracy of identification), the best results (score 2-2.3) were yielded in 55.5% of C. albicans and 67.9% of nonalbicans Candida spp. One isolate (0.2%) of non-albicans Candida spp. subsequently identified by sequencing method as Pichia fabianii was not determined (score <1.7). Biochemical typing could identify 98.6% of yeast isolates. Coincident results were received in 1287 isolates: all isolates of Candida albicans and 278 (97.5%) of non-albicans Candida spp. Exceptions were 7 isolates belonging to 4 species: C. nivariensis, C. lambica, C. famata, and Pichia fabianii.

Conclusions.

This study demonstrated a high coincidence rate for identification of yeast by MALDI-TOF MS versus conventional methods.

  • 1. Chen S.C., Marriott D., Playford E.G., et al. Candidaemia with uncommon Candida species: predisposing factors, outcome, antifungal susceptibility, and implications for management. Clin Microbiol Infect 2009; 15:662-9.
  • 2. Walsh T. J., Groll A., Hiemenz J., Fleming R., Roilides E., Anaissie E. Infections due to emerging and uncommon medically important fungal pathogens. Clin Microbiol Infect 2004; 10 (Suppl. 1):48-66.
  • 3. Miceli M.H., Díaz J.A., Lee S.A. Emerging opportunistic yeast infections. Lancet Infect Dis 2011; 11 (2):142-51.
  • 4. Мирзабалаева А.К. Инфекционные вульвовагиниты: клиническая проблема и пути ее решения. Акушерство и гинекология 2005; 6:51-5.
  • 5. Прилепская В. Н, Байрамова Г.Р. Вульвовагинальный кандидоз. Клиника, диагностика, принципы терапии. ГЭОТАР-Медиа, 2010; 80 с.
  • 6. Pfaller M.A., Diekema D. J., Gibbs D.L., et al. Results from the artemis disk global antifungal surveillance study, 1997 to 2007: 10.5-year analysis of susceptibilities of noncandidal yeast species to fluconazole and voriconazole determined by CLSI standardized disk diffusion testing. J Clin Microbiol 2009; 47:117-23.
  • 7. Sivasubramanian G., Sobel J.D. Refractory urinary tract and vulvovaginal infection caused by Candida krusei. Int Urogynecol J Pelvic Floor Dysfunct 2009; 20 (11):1379-81.
  • 8. Sanguinetti M., Porta R., Sali M., et al. Evaluation of VITEK 2 and RapID Yeast Plus systems for yeast species identification: experience at a large clinical microbiology laboratory. J Clin Microbiol 2007; 45:1343-6.
  • 9. Massonet C., Eldere J.V., Vaneechoutte M., DeBaere T., Verhaegen J., and Lagrou K. Comparison of VITEK 2 with ITS2-fragment length polymorphism analysis for identification of yeast species. J Clin Microbiol 2004; 42(5):2209-11.
  • 10. Lau A., Chen S., Sorrell T., et al. Development and clinical application of a panfungal PCR assay to detect and identify fungal DNA in tissue specimens. J Clin Microbiol 2007; 45:380-5.
  • 11. Montero C. I., Shea Y.R., Jones P.A., et al. Evaluation of pyrosequencing technology for the identification of clinically relevant non-dematiaceous yeasts and related species. Eur J Clin Microbiol Infect Dis 2008; 27:821-30.
  • 12. Alcoba-Florez J., delPilarArevalo M., Gonzalez-Paredes F.J., et al. PCR protocol for specific identification of Candida nivariensis, a recently described pathogenic yeast. J Clin Microbiol 2005; 43(12):6194-6.
  • 13. Marklein M., Josten U., Klanke E., et al. Matrix-assisted laser desorption ionization-time of flight mass spectrometry for fast and reliable identification of clinical yeast isolates. J Clin Microbiol 2009; 47(9):2912-7.
  • 14. Stevenson L.G., Drake S.K., Shea Y.R., Zelazny A.M., and Murray P.R. Evaluation of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) for the identification of clinically important yeast species. J Clin Microbiol 2010; 48:3482-6.
  • 15. Dhiman N., Hall L., Wohlfiel S.L., Buckwalter S.P., and. Wengenack N.L. Performance and cost analysis of matrix-assisted laser desorption ionization-time of flight mass spectrometry for routine identification of yeast. J ClinMicrobiol 2011; 49(4):1614-6.
  • 16. van Veen S. Q., Claas E.C., and Kuijper E. J. Highthroughput identification of bacteria and yeast by matrix-assisted laser desorption ionization time of flight mass spectrometry in conventional medical microbiology laboratories. J Clin. Microbiol 2010; 48:900-7.
  • 17. Van Herendael B. H., Bruynseels P., Bensaid M., et al. Validation of a modified algorithm for the identification of yeast isolates using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS). Eur J Clin Microbiol Infect Dis 2012; 31(5):841-8.
  • 18. Pinto A., Halliday C., Zahra M., et al. Matrix-assisted laser desorption ionization-time of flight mass spectrometry identification of yeasts is contingent on robust reference spectra. PLoS One 2011; 6(10):e25712.
  • 19. Iriart X., Lavergne R.A., Fillaux J., et al. Routine identification of medical fungi by the new Vitek MS matrixassisted laser desorption ionization-time of flight system with a new time-effective strategy. J Clin Microbiol 2012; 50 (6):2107-10.
  • 20. Анкирская А. С., Муравьева В. В., Миронова Т. Г. и соавт. Генитальный кандидоз в структуре оппортунистических инфекций влагалища. Принципы лабораторной диагностики и значение мониторинга чувствительности грибов к антимикотикам. Акушерство и гинекология 2009; 5:31-7.
  • 21. Cuenca-Estrella M., Gomez-Lopez A., Isla G., et al. Prevalence of Candida bracarensis and Candida nivariensis in a Spanish collection of yeasts: comparison of results from a reference centre and from a population-based surveillance study of candidemia. Med Mycol 2011; 49(5):525-9.
  • 22. Santos C. R., Lima N., Sampaio P., Pais C. Matrixassisted laser desorption/ionization time-of-flight intact cell mass spectrometry to detect emerging pathogenic Candida species. Diagn Microbiol Infect Dis 2011; 71(3):304-5.
  • 23. Jensen R. H., Arendrup M.C. Candida palmioleophila: characterization of a previously overlooked pathogen and its unique susceptibility profile in comparison with five related species. J Clin Microbiol 2011; 49:549-56.
  • 24. Фоменко Н. В., Иванов М. К. Разнообразие видов микроскопических грибов, выявленных в урогенитальном тракте женщин. Справочник заведующего КДЛ 2012; 11:45-53.
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