Comparison of Results of Antimicrobial Susceptibility Testing of Gram-negative Aerobic Bacteria by Disc Diffusion on Mueller-Hinton Agar and AGV Medium

Clinical Microbiology and Antimicrobial Chemotherapy. 2004; 6(2):155-167

Journal article


Results of the comparative susceptibility testing of Gram-negative aerobic bacteria by disc diffusion method on Mueller-Hinton Agar (MHA) and the AGV medium that are widely used for susceptibility testing in Russia, are presented. The aim of the study was to assess the possibility of use of AGV instead of MHA for susceptibility testing by disc diffusion. AGV was shown not to comply with WHO requirements for susceptibility testing media by several key parameters (Ca2+, Mg2+, and thymidine concentrations that are >100, >40 and >20 mg/L, respectively, whereas WHO recommends <50, <25 and <0,03 mg/L, respectively). Inconsistency of AGV composition to WHO requirements for susceptibility testing media, lack of the updated Russian recommendations for susceptibility testing and significant discrepancies of the results obtained on AGV and MHA make it impossible to use AGV for susceptibility testing of Pseudomonas aeruginosa to imipenem, aminoglycosides, and fluoroquinolones, as well as of Enterobacteriaceae to trimethoprim/sulfamethoxazole.

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