The results of identification of bacteria from positive blood cultures using MALDI-TOF mass spectrometry

Clinical Microbiology and Antimicrobial Chemotherapy. 2018; 20(4):381-386

​ Amineva P.G., Rudnov V.A., Karmatskikh O.G., Nevskaya N.N., Belsky D.V., Ivanova N.A.
10.36488/cmac.2018.4.381-386
bacteremia blood culture accelerated identification mass spectrometry
Section
Personal Experience
Type
Journal article
Publication
Clinical Microbiology and Antimicrobial Chemotherapy. 2018; 20(4):381-386

Objective.

To evaluate the results of accelerated identification of microorganisms isolated from positive hemocultures obtained from patients of various departments of a large multi-profile hospital by MALDI-TOF MS and to compare them with classical cultural methods.

Materials and Methods.

In the period from 2017 to 2018, the study included 109 positive blood cultures from 73 patients from UIA GKB number 40 at the age of 1 to 83 years. Blood samples were taken in aseptic conditions from 2 peripheral veins according to the standard protocol in 12 departments of the health facility. Identification of the grown up colonies was carried out by 2 methods: phenotypic (using the automatic bacteriological analyzer Vitek 2 (BioMerieux, France), biochemical tests, Gram stain, catalase test, oxidase, latex agglutination) in the laboratory of microbiology and MALDI-TOF MS on the Vitek MS analyzer (BioMerieux, France) in the QualityMed laboratory. The transportation of blood from the health facility to the laboratory was carried out with the help of a courier, the delivery time varied from 7 hours to 2 days. Polymicrobial hemocultures (4 vials), cases of fungemia (4 vials), and also 1 vial because of late delivery times are excluded from the study.

Results.

In total, out of 100 vials included in the study, 70 strains of Gram-positive bacteria and 29 strains of Gram-negative bacteria were isolated. Another 1 strain of Streptococcus pneumoniae was not obtained in culture due to the phenomenon of autolysis. In general, the percentage of successful accelerated identification for all groups of bacteria was 86.0%, and for gram-negative rods it reached 100%, for gram-positive cocci – 83.5%.

Conclusions.

The use of methods for accelerated identification of positive blood cultures with MALDI-TOF MS allows faster and reliable detection of pathogens of gram-negative bacteremia. The effectiveness of detection of gram-positive cocci is reduced to 83.5%.

Amineva P.G.

“QualityMed”, Yekaterinburg, Russia

Vladimir A. Rudnov

Ural State Medical University, Yekaterinburg, Russia
City Clinical Hospital №40, Yekaterinburg, Russia

Karmatskikh O.G.

“QualityMed”, Yekaterinburg, Russia

Nevskaya N.N.

City Clinical Hospital №40, Yekaterinburg, Russia

Belsky D.V.

City Clinical Hospital №40, Yekaterinburg, Russia

Ivanova N.A.

City Clinical Hospital №40, Yekaterinburg, Russia

  • 1. Kumar A., Roberts D., Wood K., et al. Duration of hypotension before initiation of effective antimicrobial therapy is the clinical determinant of survival in human septic shock. Crit Care Med. 2006;34(6):15891596.
  • 2. Ferrer R., Martin-Loeches I., Phillips G., et al. Empiric antibiotic treatment reduces mortality in severe sepsis and septic shock from the first hour: results from a guideline – based performance improvement program. Crit Care Med. 2014;42(8):1749-1755.
  • 3. Barie P., Hydo I., Shou J., et al. Influence of antibiotic therapy on mortality of critical illness caused or complicated by infection. Surg Infect. 2005;6(1):41-5
  • 4. Barochia A., Gui X., Vitberg D., et al. Bundled care for septic shock: an analysis of clinical trials. Crit Care Med. 2010;38(2):668-778.
  • 5. Galeski D., Mikkelsen M., Bard R., et al. Impact of time to antibiotics on survival in patients with severe sepsis or septic shock in whom early goal – directed therapy was initiated in emergency department. Crit Care Med. 2010;38(4):1045-1053.
  • 6. Seng P., Drancourt M., Gouriet F., et al. Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clin Infect Dis. 2009;49:543-551.
  • 7. Stevenson L.G., Drake S.K., Murray P.R. Rapid Identification of Bacteria in Positive Blood Culture Broths by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry. J Clin Microbiol. 2010;48(2):444-447.
  • 8. Fothergill A., Kasinathan V., Hyman J., et al. Rapid Identification of Bacteria and Yeasts from Positive-Blood-Culture Bottles by Using a Lysis-Filtration Method and Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrum Analysis with the SARAMIS Database. J Clin Microbiol. 2013;51(3):805-80
  • 9. Machen A., Drake T., Wang Y.F. Same Day Identification and Full Panel Antimicrobial Susceptibility Testing of Bacteria from Positive Blood Culture Bottles Made Possible by a Combined Lysis-Filtration Method with MALDI-TOF VITEK Mass Spectrometry and the VITEK2 System. PLoS One. 2014;9(2):e87870.
  • 10. La Scola B., Raoult D. Direct Identification of Bacteria in Positive Blood Culture Bottles by Matrix-Assisted Laser Desorption Ionisation Time-ofFlight Mass Spectrometry. PLoS One. 2009;4(11):e8041.
  • 11. Priputnevitch T.V., Melkumyan A.R., Burmenskaya O.V., et al. Use of MALDI-TOF Mass-Spectrometry and Quantitative PCR for Rapid Diagnosis of Sepsis. Klinicheskaja mikrobiologija i antimikrobnaja himioterapija. 2014;16(1):4-9. Russian.
  • 12. Neuville F., Descy J., Huynen P., et al. Direct identification of bacteria from BacT/ALERT anaerobic positive blood cultures by MALDI-TOF MS: MALDI Sepsityper kit versus an in-house saponin method for bacterial extraction. J Med Microbiol. 2012;61:1511-1516.
  • 13. Yonetani S., Ohnishi H., Ohkusu K., Matsumoto T., Watanabe T. Direct identification of microorganisms from positive blood cultures by MALDI-TOF MS using an in-house saponin method. Int J Infect Dis. 2016;52:37-42.
  • 14. Popov D.A., Nadtochey E.A., Vostrikova T.Yu., Ovseenko S.T. Accelerated Techniques of Pathogen Identification from Positive Blood Cultures by MALDI-TOF Mass Spectrometry. Klinicheskaja mikrobiologija i antimikrobnaja himioterapija. 2016;18(4):296-307. Russian.
  • 15. Kok J., Thomas L.C., Olma T., Chen S.C.A., Iredell J.R. Identification of Bacteria in Blood Culture Broths Using Matrix-Assisted Laser Desorption-Ionization Sepsityper and Time of Flight Mass Spectrometry. PLoS One. 2011;6(8):e23285.
  • 16. Popov D.A., Ovseenko S.T., Vostrikova T.Yu. Express identification of positive blood cultures using direct MALDI-TOF mass spectrometry. Anesteziologiya i reanimatologiya. 2015;60(5):71-75. Russian.
  • 17. Vincent J-L., Moreno R., Takala J., et al. The SOFA (Sepsis-Related Organ Failure Assessment) score to describe organ dysfunction/ failure. Intensive Care Med. 1996;22(7):707-710.
  • 18. Ferreira L., Sánchez-Juanes F., Munoz-Bellido J.L., González-Buitrago J.M. Rapid method for direct identification of bacteria in urine and blood culture samples by matrix-assisted laser desorption ionization time-of-flight mass spectrometry: intact cell vs. extraction method. Clin Microbiol Infect. 2011;17:1007-1012.
  • 19. Jakovljev A., Bergh K. Development of a rapid and simplified protocol for direct bacterial identification from positive blood cultures by using matrix assisted laser desorption ionization time-of- flight mass spectrometry. BMC Microbiol. 2015;15:258.
  • 20. Sung J.J., Park K.G., Han K., Park D.J., Park Y.J. Direct Identification and Antimicrobial Susceptibility Testing of Bacteria From Positive Blood Culture Bottles by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry and the Vitek 2 System. Ann Lab Med. 2016;36:117-123.
  • 21. Angeletti S., Dicuonzo G., D’Agostino A., et al. Turnaround time of positive blood cultures after the introduction of matrix-assisted laser desorption-ionization time-of-flight mass spectrometry. New Microbiol. 2015;38:379-386.
  • 22. Schieffer K.M., Tan K.E., Stamper P.D., et al. Multicenter evaluation of the SepsityperTM extraction kit and MALDI-TOF MS for direct identification of positive blood culture isolates using the BD BACTECTM FX and VersaTREK diagnostic blood culture systems. J Appl Microbiol. 2014;116:934-941.
  • 23. Ruiz-Aragón J., Ballestero-Téllez M., Gutiérrez-Gutiérrez B., et al. Direct bacterial identification from positive blood cultures using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry: A systematic review and meta-analysis. Enferm Infecc Microbiol Clin. 2018;36(8):484-492.
  • 24. Chien J.-Y., Lee T.-F., Du S.-H., et al. Applicability of an in-House Saponin-Based Extraction Method in Bruker Biotyper Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry System for Identification of Bacterial and Fungal Species in Positively Flagged Blood Cultures. Front Microbiol. 2016;7:1432.
  • 25. Lin J., Ge M., Liu T., Chang S., Lu J. A simple method for rapid microbial identification from positive monomicrobial blood culture bottles through matrix-assisted laser desorption ionization time-of-flight mass spectrometry. J Microbiol Immunol Infect. 2018;51(5):659-665.
  • 26. Morgenthaler N. G., Kostrzewa M. Rapid Identification of Pathogens in Positive Blood Culture of Patients with Sepsis: Review and MetaAnalysis of the Performance of the Sepsityper Kit. Int J Microbiol. 2015;827416:10.
  • 27. Tholpady S.S., Sifri C.D., Sawyer R.G., et al. Leuconostoc pseudomesenteroides blood stream infection following liver transplantation. Ann Transplant. 2010;15(1):61-66.
  • 28. Florescu D., Hill L., Sudan D., Iwen P. C. Leuconostoc Bacteremia in Pediatric Patients With Short Bowel Syndrome: Case Series and Review. Pediatr Infect Dis J. 2008;27(11):1013-1019.
  • 29. Horii T., Notake S., Tamai K., Yanagisawa H. Bacillus cereus from blood cultures: virulence genes, antimicrobial susceptibility and risk factors for blood stream infection. FEMS Immunol Med Microbiol. 2011;63:202-209.
  • 30. Bab'eva I.P., Chernov I.Ju. Yeasts biology. M.: 2004, p. 175-176. Russian.
  • 31. Lockhart S.R., Messer S.A., Pfaller M.A., Diekema D.J. Lodderomyces elongisporus Masquerading as Candida parapsilosis as a Cause of Bloodstream Infections. J Clin Microbiol. 2008;46(1):374-376.
  • 32. Ahmad S., Khan Z. U., Johny M., et al. Isolation of Lodderomyces elongisporus from the Catheter Tip of a Fungemia Patient in the Middle East Publishing Corporation. Case Rep Med. 2013;560406:5.
  • 33. Hatanaka S., Nakamura I., Fukushima S., Ohkusu K., Matsumoto T. Catheter-Related Bloodstream Infection Due to Lodderomyces elongisporus. J Infect Dis. 2016;69:520-522.
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