mass-spectrometry

Disseminated Mycobacterium genavense infection in an otherwise healthy child: a clinical case and literature review

This paper presents the first clinical case of combined pulmonary and abdominal M. genavense infection in an otherwise healthy, immunocompetent child with an early treatment response and full recovery. The favorable outcome was determined by rapid …

The results of identification of bacteria from positive blood cultures using MALDI-TOF mass spectrometry

Objective. To evaluate the results of accelerated identification of microorganisms isolated from positive hemocultures obtained from patients of various departments of a large multi-profile hospital by MALDI-TOF MS and to compare them with classical cultural methods. Materials and Methods. In the period from 2017 to 2018, the study included 109 positive blood cultures from 73 patients from UIA GKB number 40 at the age of 1 to 83 years. Blood samples were taken in aseptic conditions from 2 peripheral veins according to the standard protocol in 12 departments of the health facility.

Use of the MALDI Biotyper system and the microbiological diagnosis algorithm for identification of non-fermenting bacteria isolated from respiratory tract in cystic fibrosis patients

The objective of this study was to compare the MALDI Biotyper system and the microbiological diagnosis algorithm for identification of non-fermenting bacteria isolated from respiratory tract in cystic fibrosis patients. The identification and typing …

Nontuberculous mycobacteria: modern possibilities of species identification

The paper presents up-to-date review on existing methods of identification of nontuberculous mycobacteria. The advantages and limitations of the use of different identification methods in the real practice in specialized TB laboratories and general …

Lactobacilli Species Diversity in Different States of Vaginal Microbiota in Pregnant Women

Objective. To determine lactobacilli species diversity in different states of vaginal microbiota in pregnant women using culture, mass-spectrometry, and DNAbased assays. Materials and Methods. A total of 163 pregnant women (18 to 40 years of age) were examined in the 2nd or 3rd trimester. A comprehensive microbiological examination of vaginal swabs, including Gram staining, methylene blue staining, and culture was performed. All isolated microorganisms were identified to species level using mass-spectrometry.