Personal Experience

Objective. To evaluate the dynamics of antimicrobial resistance of Escherichia coli isolates from the urine of patients hospitalized in medical institutions of Tashkent city from 2018 to 2021. Materials and Methods. The study included 1020 E. coli isolates from the urine of patients with clinical symptoms of urinary tract infections admitted to the reference laboratory of the Antimicrobial Resistance Research Center (ARRC) from three medical institutions in Tashkent city for the period 2018– 2021.

Objective. To identify specific features of the clinical course of Congo-Crimean hemorrhagic fever (CCHF) in children in order to optimize early and differential diagnosis, management tactics and treatment of pediatric patients Materials and Methods. A retrospective analysis of 159 probable and confirmed cases of Congo-Crimean hemorrhagic fever was carried out in patients admitted to the infectious diseases departments of Shymkent, district hospitals of Turkestan region for the period 2013–2022, of which 39 (24.

Objective. To determine the level of knowledge of doctors on the rational use of antimicrobial drugs in clinical practice and to compare with data of the previous stage of the study and results obtained from students in a parallel project. Materials and Methods. A multicenter questionnaire survey of practitioners with analysis of results – stage of KANT project (full name of the project – «Physicians’ (Students’) knowledge in antimicrobials usage») conducted in 2020–2023.

Objective. The aim of the study was to develop gradient diffusion strips with substances capable of potentiating the action of antibiotics for the treatment of infections caused by multidrug-resistant strains of K. pneumoniae. Materials and Methods. The substances azidothymidine and baicalin were used to evaluate the joint action of combinations of antibiotics with substances that potentiate their action. Determination of susceptibility to combinations of antibiotics (gentamicin, cefotaxime, ciprofloxacin and chloramphenicol) with the listed substances was carried out by a modified gradient-diffusion method (cross-test).

Objective. To determine the level of basic knowledge of 5–6-year medical students in the issues of appropriate antimicrobial therapy. Materials and Methods. A multicenter questionnaire survey with analysis of results – stage of KANT project (full name of the project – «Physicians’ (Students’) knowledge in antimicrobials usage») conducted in 2020-2023. Results. A total of 277 students in 6 regions of Russia were surveyed. The average level of correct answers for the whole questionnaire amounted to 42.

Objective. To develop the method for determining the viability of M. leprae using polymerase chain reaction to evaluate the effectiveness of treatment. Materials and Methods. 54 scarifications and 10 biopsies of the skin of patients with leprosy were studied. Ribosomal 16S rRNA genes were used as a target. Results. The high sensitivity and specificity of the developed real-time reverse transcription PCR method was established. M. leprae were detected on average 2 times more often by RT-PCR compared with the bacterioscopy method both before treatment and after its six-month course (p < 0.

Objective. To conduct a comparative evaluation of the results of phenotypic and molecular genetic methods for testing drug resistance of Mycobacterium tuberculosis isolated from bone biopsies of patients with tuberculous spondylitis. Materials and Methods. A retrospective cohort study was conducted on the case histories of patients diagnosed with tuberculous spondylitis who underwent surgical treatment in the period from 2016 to 2021. The main study subject was surgical material. Antimicrobial resistance patterns of MBT isolates was performed using phenotypic and molecular genetic methods.

Objective. To study significance of herpesviruses in the development of infectious complications in patients after heart transplantation based on the detection of markers of herpesvirus infections. Materials and Methods. The paper presents the results of a comprehensive examination for markers (HVI) of patients who underwent orthotopic heart transplantation at the V.I. Shumakov National Medical Center of Transplantology and Artificial Organs (Moscow). A total of 86 patients were observed. To establish the etiological diagnosis of infectious complications, all the blood serum and blood cell samples were analyzed for markers of herpesvirus infections.

Objective. To compare results of microbiological examination obtained on rapid automated system with semiquantitative plate culture to assess possibility and necessity of using the system in urine examination. Materials and Methods. The study included 231 urine samples collected from February to July 2023 from patients at Lomonosov Moscow State University Medical Research and Educational Center. The samples were cultured according to the standards of urine microbiological examination using chromogenic media and using an automatic HB&L system (Alifax, Italy) for 4 h.

Objective. To study the real practice of management of patients with COPD exacerbations on the example of a single hospital. Materials and Methods. During the retrospective descriptive study the data of medical records of 219 patients with COPD were processed, the profile of the studied patients with distribution by phenotypes and determination of the Charlson comorbidity index was compiled and analyzed. StatPlus 7.0 program was used for statistical analysis.