Personal Experience

Objective. To assess pathogen identification from positive blood cultures by MALDI-TOF mass spectrometry. Materials and Methods. At the first stage, an in vitro study using MALDI-TOF MS and including 11 pathogen species (K. pneumoniae, E. coli, P. aeruginosa, A. baumannii, S. aureus, S. epidermidis, S. haemolyticus, E. faecalis, E. faecium, C. albicans, C. parapsilosis) was performed. This study involved hourly attempts to identify the above listed pathogens by MALDI-TOF MS (Vitek MS, bioMerieux) during incubation until their correct identification.

This article describes a clinical case of fatal polymicrobial pneumonia in a patient with progressive pulmonary tuberculosis. The clinical case represents a co-infection with tuberculosis, viral and pneumocystis infection in a HIV-uninfected patient …

This paper presents results of the study aimed to evaluate level of knowledge on antibacterial therapy and empiric use of antimicrobial agents in the treatment of respiratory infections in medical students. A total of 197 fifth and sixth year medical …

Objective. To characterize gut microbiota composition in patients with different body weight. Materials and Methods. A total of 92 patients (mean age: 52.8 years) with different body mass index (BMI), with or without abdominal obesity were enrolled in the study. A metagenomic analysis as an assessment of 16S rRNA gene diversity in a total DNA sample was performed according to 16S Metagenomic Sequencing Library Preparation protocol. Taxonomic composition of the samples was evaluated using 16S rRNA gene sequences database Greengenes v.

Topical management of wounds and wound infections is still the area of surgery in which there are no clear standards. A number of topical antimicrobial agents are used in clinical practice as a result of deficiency of adequate information in surgical …

We studied 126 children, admitted to cardiac surgery hospital with colonization of the gastrointestinal tract (GIT) by multidrug-resistant Enterobacteriaceae. Criterion for inclusion in the study were children with complex congenital heart disease …

This paper presents the results of a microbiological study of clinical specimens obtained from 64 patients with chronic post-traumatic osteomyelitis of long bones during the 2011–2013. A total of 136 clinical strains were isolated and identified to a …

A total of 717 serum samples from haematological patients, who were hospitalized in Botkin City Clinical Hospital (Moscow) in the 2011–2014, were tested. High frequency of hepatitis B virus (HBV) serologic markers was established: HBsAg — 4.5%; …

Objective. To assess potential role of opportunistic microorganisms in the etiology of chronic endometritis by comparison of microbiota of upper and lower genital tract in women of reproductive age with different histopathologic findings of chronic endometritis and endometrial polyps. Materials and Methods. A total of 164 women of reproductive age were enrolled in this study and assigned to four groups by histopathologic changes in endometrium: I — complete set of chronic endometritis findings (n=38); II — partial set of chronic endometritis findings (n=92); III — endometrial polyps (n=15); IV — control group (n=19).

This article presents current view about the mechanisms of human gut microbiota influence on chronic systemic inflammation. Over the last years, evidence of close relationship between different medical conditions and gut microbiota status has been …