molecular typing

Genetic antimicrobial resistance determinants and their prevalence in molecular subtypes of Treponema pallidum subsp. pallidum

Objective. To investigate genetic determinants of resistance to antimicrobial agents recommended for the treatment of syphilis and assess their prevalence in molecular subtypes of Treponema pallidum subsp. pallidum in the Russian Federation over the period of 2014-2017. Materials and Methods. A total of 161 clinical isolates of T. pallidum obtained from Tyva, Stavropol, Irkutsk, Kaluga, Novosibirsk and Omsk regions were included in this study. Genetic material of T. pallidum was detected by PCR with primers to polA gene.

Molecular Epidemiology of Staphylococcus aureus in Russian Pediatric Hospitals

Objective. To reveal molecular types of nosocomial and community-acquired strains of S. aureus in different regions of Russia. Materials and Methods. 575 (187 MRSA and 388 MSSA) strains, isolated from pediatric patients during multicenter studies (1997–2008) from 36 medical institutions in 24 Russian cities were included in the study. For molecular typing 186 strains with unique resistance profile within each ward of each medical institution were selected. The following typing methods were used: MLVA, spa, SCCmec and MLST.

MRSA Typing: Which Methods are the Most Appropriate for Different Purposes?

Over the last decades there is an uncontrollable rise of MRSA prevalence. Infections caused by MRSA are associated with high mortality and healthcare costs. New antimicrobial drugs (anti-MRSA cephems, new glycopeptides, lipopeptides, glycilcyclines …

Genetic Characteristics of Neisseria meningitidis Strains in Moscow

This paper provides data on genetic characteristics of circulating Neisseria meningitidis strains in Moscow over the period of 2001–2010. Multilocus sequence typing (MLST) method was used according to international standards. Retrospective analysis …

Use of Laboratory Standards for Diagnosis of Legionellosis during the Outbreak of Pneumonia in Verhnyaya Pyshma (Sverdlovsk Region)

Over the last years there was a significant improvement in diagnostics and prophylaxis of legionellosis that is due to introduction of current standards of laboratory diagnosis and quantitative analysis of the pathogen in the environmental objects. …