Abstract
Polymerase chain reaction (PCR) and restriction fragment length polymorphisms (RFLP) analysis revealed polymorphism of prfA and plcA genes, encoding the pathogenecity factors in strains of Listeria monocytogenes. Phylogenetic analysis confirmed the presence of at least two distinct evolutionary L. monocytogenes lines, existing of which was suggested in a number of studies based on analysis of other chromosomal DNA sites [1, 4]. There was shown to be a predominance of certain virulence,associated genes polymorphisms depending on type of sample (hospital environment, foods). The L. monocytogenes strains with a large number of sequence differences in this region (smaller than expected size of targeted DNA fragment or its absence) were found to be avirulent.
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