Listeria monocytogenes

Distribution of antimicrobial resistance among clinical and food Listeria monocytogenes isolated in Moscow in 2019–2021

Objective. To determine the current state-of-art of acquired resistance to antimicrobial drugs among L. monocytogenes strains associated with listeriosis in humans and food contamination in Moscow. Materials and Methods. We used 39 L. monocytogenes strains isolated in Moscow in 2019–2021 from clinical material (n = 24) and food (n = 15). Resistance to 12 antibiotics of the first and second lines of defense was studied using disk-diffusion method. The parameters recommended for L.

Listeriosis: genotyping as a key for identification a possible source of infection

Objective. To perform a comparative analysis of clinical and food isolates of Listeria monocytogenes collected in the European part of Russia in 2018–2019. Materials and Methods. We used multilocus sequencing (MLST), supplemented by virulence loci, including fragments of internalin genes (MvLST, Multi-virulent-locus sequence typing), followed by phylogenetic analysis. Results. The main diagnoses for clinical isolates were prenatal and neonatal listeriosis and meningitis. Clinical isolates predominantly belonged to phylogenetic line II with the predominance of ST7, which was also the most abundant in food isolates.

Molecular Genetic Characteristics and Epidemiological Significance of Listeria monocytogenes Isolated from Pregnant Women and Aborted Fetuses in the Far East Region of Russia

L. monocytogenes strains isolated from aborted fetuses (n=13) and pregnant women (n=4) in the Far East region of Russia were studied. Of 17 tested strains, 11 were serovar 4b, 5 – serovar 1/2a and 1 – serovar 1/2b. Based on the sequence of inlA, inlB …

Typing of Listeria monocytogenes on the Basis of Polymorphism of Genes Encoding the Pathogenecity Factors

Polymerase chain reaction (PCR) and restriction fragment length polymorphisms (RFLP) analysis revealed polymorphism of prfA and plcA genes, encoding the pathogenecity factors in strains of Listeria monocytogenes. Phylogenetic analysis confirmed the …

New Methods for Identification of Listeria monocytogenes

Identification of Listeria monocytogenes in clinical specimens is often complicated because of morphological and biochemical properties of this pathogen. In present article the traditional bacteriological methods of identification of L.monocytogenes …