Clinical Microbiology and Antimicrobial Chemotherapy. 2001; 3(3):266-273
Identification of Listeria monocytogenes in clinical specimens is often complicated because of morphological and biochemical properties of this pathogen. In present article the traditional bacteriological methods of identification of L.monocytogenes are compared with PCR and original method of identification based on induction of lecithinase activity in the presence of activated charcoal. In the PCR with specific to the gene plcA primers there was 100% specificity and high sensitivity. At the same time in 89% of L.monocytogenes strains the specific induction of lecithinase activity by activated charcoal has been shown, when no such a phenomenon have been observed in non-monocytogenes Listeria and other bacteria studied.