Polymerase chain Reaction

Objective. To develop the method for determining the viability of M. leprae using polymerase chain reaction to evaluate the effectiveness of treatment. Materials and Methods. 54 scarifications and 10 biopsies of the skin of patients with leprosy were studied. Ribosomal 16S rRNA genes were used as a target. Results. The high sensitivity and specificity of the developed real-time reverse transcription PCR method was established. M. leprae were detected on average 2 times more often by RT-PCR compared with the bacterioscopy method both before treatment and after its six-month course (p < 0.

Polymerase chain reaction (PCR) and restriction fragment length polymorphisms (RFLP) analysis revealed polymorphism of prfA and plcA genes, encoding the pathogenecity factors in strains of Listeria monocytogenes. Phylogenetic analysis confirmed the …

Today’s medicine takes advantage of the latest achievements in natural sciences and successfully employs new techniques to the diagnostics and treatment of diseases. Recently molecular-genetic approaches have been applied in the field of …