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Personal Experience

Pharmacoepidemiology of antimicrobial agents in pregnant women: a non-interventional retrospective study

The present study was conducted within the framework of Saint Petersburg’s multi-purpose programmes initiated in 2014 to restrain antimicrobial resistance. The study was observational and retrospective. The analysis included three maternity …

Compliance of paediatricians to the guidelines on the use of antimicrobials in respiratory infections

Objective. To monitor the level of compliance of paediatricians regarding antimicrobial therapy of acute respiratory infections in children. Materials and Methods. Аnonymous survey of paediatricians of Rostov region was conducted using a questionnaire developed by the authors during 2009-10 and 2014-15. The analysis was performed to assess the compliance with the national recommendations developed by the Union of paediatricians of Russia among the 3 groups of doctors (practicing ≤5 years; 6-20 years; ≥21 years).

Real-Time PCR for the Diagnosis of Tick-Born Infections

Overall 842 cases of admission due to tick bites in Irkutsk area during 2014 were analyzed. Detection of DNA/RNA of the tick-born encephalitis virus, Lyme borreliosis, monocytic ehrlichiosisand granulocyticanaplasmosis in the human serum and in the …

Accelerated Techniques of Pathogen Identification from Positive Blood Cultures by MALDI-TOF Mass Spectrometry

Objective. To assess pathogen identification from positive blood cultures by MALDI-TOF mass spectrometry. Materials and Methods. At the first stage, an in vitro study using MALDI-TOF MS and including 11 pathogen species (K. pneumoniae, E. coli, P. aeruginosa, A. baumannii, S. aureus, S. epidermidis, S. haemolyticus, E. faecalis, E. faecium, C. albicans, C. parapsilosis) was performed. This study involved hourly attempts to identify the above listed pathogens by MALDI-TOF MS (Vitek MS, bioMerieux) during incubation until their correct identification.

A Clinical Case of Acute Herpesvirus and Pneumocystis Co-infection in a Patient with Progressive Pulmonary Tuberculosis

This article describes a clinical case of fatal polymicrobial pneumonia in a patient with progressive pulmonary tuberculosis. The clinical case represents a co-infection with tuberculosis, viral and pneumocystis infection in a HIV-uninfected patient …

Awareness of Antimicrobial Chemotherapy in Fifth and Sixth Year Medical Students

This paper presents results of the study aimed to evaluate level of knowledge on antibacterial therapy and empiric use of antimicrobial agents in the treatment of respiratory infections in medical students. A total of 197 fifth and sixth year medical …

Gut Microbiota Composition in Patients with Different Body Weight

Objective. To characterize gut microbiota composition in patients with different body weight. Materials and Methods. A total of 92 patients (mean age: 52.8 years) with different body mass index (BMI), with or without abdominal obesity were enrolled in the study. A metagenomic analysis as an assessment of 16S rRNA gene diversity in a total DNA sample was performed according to 16S Metagenomic Sequencing Library Preparation protocol. Taxonomic composition of the samples was evaluated using 16S rRNA gene sequences database Greengenes v.

Topical Treatment of Wounds and Wound Infection: Results of Anonymous Surgeons Questioning

Topical management of wounds and wound infections is still the area of surgery in which there are no clear standards. A number of topical antimicrobial agents are used in clinical practice as a result of deficiency of adequate information in surgical …

Multidrug-Resistant Enterobacteriaceae Colonizing Gastrointestinal Tract in Infants with Congenital Heart Disease on Admission to Cardiac Surgery Hospital

We studied 126 children, admitted to cardiac surgery hospital with colonization of the gastrointestinal tract (GIT) by multidrug-resistant Enterobacteriaceae. Criterion for inclusion in the study were children with complex congenital heart disease …

Antimicrobial Resistance of Microorganisms Causing Chronic Post-Traumatic Osteomyelitis

This paper presents the results of a microbiological study of clinical specimens obtained from 64 patients with chronic post-traumatic osteomyelitis of long bones during the 2011–2013. A total of 136 clinical strains were isolated and identified to a …