Staphylococcus aureus

Cefazolin inoculum effect among methicillinsusceptible Staphylococcus aureus isolated from patients with skin infections

Objective. To evaluate frequency and intensity of cefazolin inoculum effect among methicillin-susceptible staphylococci isolated from patients with skin infections. Materials and Methods. A total of 80 methicillin susceptible isolates of Staphylococcus aureus were identified by cefoxitin disk-diffusion test and negative results of real-time PCR for mecA gene. Inoculum effect was measured by broth microdilution test with two inocula with concentrations of 5 × 105 CFU/mL and 5 × 107 CFU/mL.

Unresolved issues of antibiotic therapy of infections caused by Staphylococcus aureus

Staphylococcal infections are widespread among outpatients and hospitalized patients. Despite the apparent diversity of the “menu” of anti-staphylococcal drugs, treatment results in several patients are not good as expected. This is due to the …

Antimicrobial resistance of nosocomial Staphylococcus aureus isolates in Russia: results of multicenter epidemiological study «MARATHON» 2013-2014

The study included 418 of the 841 clinical isolates of S. aureus, which were regarded as nosocomial. These 418 clinical nosocomial S. aureus strains were isolated in 2013-2014 from patients hospitalized in 25 hospitals in 19 Russian cities. …

Antimicrobial Resistance of Microorganisms Causing Chronic Post-Traumatic Osteomyelitis

This paper presents the results of a microbiological study of clinical specimens obtained from 64 patients with chronic post-traumatic osteomyelitis of long bones during the 2011–2013. A total of 136 clinical strains were isolated and identified to a …

Antimicrobial Resistance of Nosocomial Staphylococcus aureus Isolates in Russia: Results of National Multicenter Surveillance Study «MARATHON» 2011–2012

In total 284 clinical nosocomial S. aureus strains isolated in 2011–2012 from patients hospitalized in 25 hospitals in 18 Russian cities were included in the study. Susceptibility to 19 antimicrobials was assessed by serial dilution method in …

Genotyping of Staphylococcus aureus Isolates in Outbreak of Exfoliative Dermatitis in Neonates

Staphylococcus aureus is known as the leading pathogen in hospital-acquired infections and the most common cause of skin and soft tissues. The present study aimed to test the genetic background of MSSA isolates obtained from pediatric patients and …

A New Method for Evaluation of Antimicrobial Resistance of Bacteria in Biofilms

At the present article we evaluate a relatively new method of the evaluation of activity of antimicrobials against bacterial biofilms. This method combines the possibilities of simultaneous evaluation of bacterial survival in biofilm in the presence …

Identification of a Novel MRSA ST239 Strain Producing Toxic Shock Syndrome Toxin (TSST-1) in a HIV-infected Patient with Pneumonia

Methicillin-resistant Staphylococcus aureus (MRSA) ST239 is one of the most common nosocomial genotypes worldwide. Typical MRSA ST239 strains do not produce toxic shock syndrome toxin-1 (TSST-1), which is encoded by tst gene at staphylococcal …

Detection of Hungarian Pandemic MRSA Clone in Russia

Since 1961, pandemic methicillin-resistant S. aureus (MRSA) clones causing severe nosocomial infections have been distributed worldwide. The Hungarian clone having ST239 genotype and Staphylococcal cassette chromosome mec (SCCmec) type III is one of …

Prediction of Development of Antimicrobial Resistance in In-vitro Dynamic Models of Different Dosage Regiments

According to the mutant selection window (MSW) hypothesis resistant mutants are selected at antibiotic concentrations above the MIC but below the mutant prevention concentration (MPC). There are contradictory reports on the relationships between MIC- …