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Objective. To perform phylogenetic analysis to assess ongoing changes in population structure of Russian clinical isolates of N. gonorrhoeae. Materials and Methods. The object of the study was 34 clinical isolates of N. gonorrhoeae obtained from dermatovenerological medical institutions of the Russian Federation in the period 2022–2023. The cultures were spread on chocolate agar with 1% ISOVitalex growth additive and 1% VCAT selective additive (Becton Dickinson, USA). Mass spectrometric studies were performed using MALDI-TOF mass spectrometer (Bruker Daltonics, Germany).

Objective. To study in vitro activity of antimicrobials against clinical isolates obtained from patients with community-acquired urinary tract infections (UTIs) in various regions of Russia in 2023. Materials and Methods. The study included 1098 isolates obtained from the urine of children and adults of both sexes of all age groups with acute and exacerbation of recurrent community-acquired UTIs, including pregnant women with asymptomatic bacteriuria. Isolates were collected in 29 centers in 21 cities of Russia in 2023 as part of a multicenter prospective epidemiological study of the dynamics of antibiotic resistance of pathogens causing community-acquired UTIs in different subsets of patients («DARMIS-2023»).

Objective. To evaluate in vitro activity of various macrolide antibiotics against a current collection of clinical isolates of Streptococcus pneumoniae and Streptococcus pyogenes isolated from patients with community-acquired infections in different regions of the Russian Federation. Materials and Methods. A total of 350 clinical isolates from patients with community-acquired infections, including 200 S. pneumoniae and 150 S. pyogenes, were included in the study. To further evaluate the in vitro activity of 16-member macrolides against erythromycin-resistant isolates (MIC ≥ 0.

Objective. In 2023–2014, a significant increase in the incidence of pneumonia caused by M. pneumoniae has been reported in Russia and China. Despite being one of the primary causative agents of community-acquired lower respiratory tract infections in children, due to the difficulties in obtaining a culture of this pathogen, the amount of genomic data on M. pneumoniae is currently extremely limited, with fewer than 300 genomic sequences available, of which only 79 are complete.

Objective. To evaluate the dynamics of antimicrobial resistance of Escherichia coli isolates from the urine of patients hospitalized in medical institutions of Tashkent city from 2018 to 2021. Materials and Methods. The study included 1020 E. coli isolates from the urine of patients with clinical symptoms of urinary tract infections admitted to the reference laboratory of the Antimicrobial Resistance Research Center (ARRC) from three medical institutions in Tashkent city for the period 2018– 2021.

Objective. To identify specific features of the clinical course of Congo-Crimean hemorrhagic fever (CCHF) in children in order to optimize early and differential diagnosis, management tactics and treatment of pediatric patients Materials and Methods. A retrospective analysis of 159 probable and confirmed cases of Congo-Crimean hemorrhagic fever was carried out in patients admitted to the infectious diseases departments of Shymkent, district hospitals of Turkestan region for the period 2013–2022, of which 39 (24.

Objective. To determine the level of knowledge of doctors on the rational use of antimicrobial drugs in clinical practice and to compare with data of the previous stage of the study and results obtained from students in a parallel project. Materials and Methods. A multicenter questionnaire survey of practitioners with analysis of results – stage of KANT project (full name of the project – «Physicians’ (Students’) knowledge in antimicrobials usage») conducted in 2020–2023.

Objective. To study epidemiology of resistance to macrolides and fluoroquinolones in Mycoplasmagenitalium isolated from pregnant women in Russia and Belarus. Materials and Methods. A total of 107 M. genitalium DNA samples isolated from pregnant women in Russia (n = 58) and Belarus (n = 49) was included in the study. In order to detect markers of macrolide or fluoroquinolone resistance, polymerase chain reaction in real time with the effect of quenching fluorescence of the probe by a primer was used.

Objective. To study the dynamics and molecular mechanisms of resistance of Streptococcus agalactiae strains isolated from pregnant women and newborns in St. Petersburg in 2010–2022. Materials and Methods. The strains of S. agalactiae (n = 248) were obtained by analyzing the clinical material of pregnant women and newborns who were hospitalized at Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott in 2010–2011 and 2020–2022. Determination of susceptibility to antibacterial drugs was evaluated by disk diffusion method.

Objective. To study the antimicrobial susceptibility profile of Moraxella catarrhalis isolates from different regions of Russia in 2010–2021. Materials and Methods. The study included 352 isolates of M. catarrhalis isolated in 11 cities of Russia (Volgograd, Irkutsk, Kazan, Kirov, Moscow, Murmansk, Perm, Seversk, Togliatti, Tomsk, and Ulan-Ude) in 2010–2021. Identification was done by MALDI-TOF mass spectrometry. Antimicrobial susceptibility testing was determined by broth microdilution method in accordance with the ISO 20776-1:2020.