Current methods of capsular typing of Streptococcus pneumoniae: possibilities and availability for local laboratories | CMAC

Current methods of capsular typing of Streptococcus pneumoniae: possibilities and availability for local laboratories

Clinical Microbiology and Antimicrobial Chemotherapy. 2022; 24(1):61-66

​ Chagaryan A.N., Ivanchik N.V., Mironov K.O., Muravyev A.A.
10.36488/cmac.2022.1.61-66
Streptococcus pneumoniae cps-locus serotyping serological methods real-time PCR whole genome sequencing
Section
Microbiological Diagnostics
Type
Review
Publication
Clinical Microbiology and Antimicrobial Chemotherapy. 2022; 24(1):61-66

Abstract

Pneumococcal conjugate vaccines contain a limited number of serotype-specific antigens of S. pneumoniae. It is important for vaccination programmes effectiveness assessment to control a variety of circulating S. pneumoniae serotypes. This review provides an analysis of pneumococcal serotyping approaches and further ways of improving pneumococcal serotype detection within the microbiological surveillance. Serological methods and multiplex PCR can identify a limited number of pneumococcal serotypes only. Whole-genome sequencing-based approaches can predict almost all serotypes and sequence types as well as detect antimicrobial resistance and virulence genes.

Aida N. Chagaryan

Institute of Antimicrobial Chemotherapy, Smolensk, Russia

Nataly V. Ivanchik

Institute of Antimicrobial Chemotherapy, Smolensk, Russia

Konstantin O. Mironov

Central Research Institute of Epidemiology, Moscow, Russia

Alexander A. Muravyev

Institute of Antimicrobial Chemotherapy, Smolensk, Russia

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