real-time PCR

Objective. To develop and conduct the first stage of validation for a PCR test that identifies key mutations in the V domain of the 23S rRNA gene of M. pneumoniae (A2058G, A2059G, and A2062C). This test allows for the identification of macrolide-resistant M. pneumoniae strains in samples of native biological material. Materials and Methods. The study included a panel of M. pneumoniae DNA samples (n = 107) from the collection of the DeMaRes multicenter study, as well as native material (n = 52).

Pneumococcal conjugate vaccines contain a limited number of serotype-specific antigens of S. pneumoniae. It is important for vaccination programmes effectiveness assessment to control a variety of circulating S. pneumoniae serotypes. This review …

A clinical case of acute epididymitis in a 5-month male infant is presented. Taking into account the occurrence of epididymitis in infancy, an intra-operative biopsy sample was tested for multiple viral pathogens by real-time polymerase chain …

Over the last years there was a significant improvement in diagnostics and prophylaxis of legionellosis that is due to introduction of current standards of laboratory diagnosis and quantitative analysis of the pathogen in the environmental objects. …