whole genome sequencing

Objective. Conducting a whole-genome study and bioinformatic analysis of a Lacticaseibacillus strain to confirm taxonomic affiliation, identify genetic determinants of metabolic activity, stress resistance, and bacteriocin production as a criterion for the standardization of mono- and multi-component probiotics. Materials and Methods. The object of the study was a strain isolated from a coloproctology patient in 2024. Cultivation was carried out on MRS medium under microaerophilic conditions. Wholegenome sequencing was performed on the Oxford Nanopore Technologies MinION platform (R10.

Objective. To study genetic determinants of resistance and virulence of K. pneumoniae strains received by the reference center for the prevention of the spread of biological threats of the National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation. Materials and Methods. The study included 153 strains of Klebsiella pneumoniae isolated from the biological material of pregnant women and newborns in Perinatal centers in various regions of the Russian Federation with infectious and inflammatory diseases and included in the collection of opportunistic microorganisms of the reference center in 2023.

Objective. Seroyping of the Streptococcus pneumoniae strains obtained from patients with invasive and non-invasive forms of pneumococcal infection circulating in Russia using whole-genome sequencing. Materials and Methods. The 144 invasive and 90 non-invasive S. pneumoniae strains isolated in 2002– 2023 during the multicenter epidemiological study «PEGASus» were investigated. Real-time PCR and whole-genome sequencing were used to determine serotypes. The 16 serotyping targets were examined by PCR technique. Whole-genome sequencing was performed on the Illumina equipment.

Pneumococcal conjugate vaccines contain a limited number of serotype-specific antigens of S. pneumoniae. It is important for vaccination programmes effectiveness assessment to control a variety of circulating S. pneumoniae serotypes. This review …

Objective. To present the data on the main mechanism of molecular variation in P. aeruginosa causing chronic lung infection in patients with cystic fibrosis. Materials and Methods. A total of 1800 throat swabs and sputum samples from cystic fibrosis patients were included in the study over the 10-year period. P. aeruginosa isolates were primarily identified by the biochemical method using the API 20NE test strips (bioMerieux, France). Antimicrobial susceptibility testing was performed by disc diffusion method.

In order to determine molecular mechanisms of resistance to ciprofloxacin, clinical isolates of Mycoplasma hominis (2 strains) obtained from women with pelvic inflammatory disease were investigated. Whole genome sequencing was performed using a …