Abstract
In order to determine molecular mechanisms of resistance to ciprofloxacin, clinical isolates of Mycoplasma hominis (2 strains) obtained from women with pelvic inflammatory disease were investigated. Whole genome sequencing was performed using a high-performance MiSeq sequencer (Illumina, USA). M. hominis M57 and M45 isolates were found to have the proportion of GC-bases which is typical for this species (27.2%). A high degree of the protein sequences homology of the M57 and M45 isolates has been determined. Phylogenetic analysis showed that the M57 isolate is evolutionarily closer to the PG21 isolate, and M45 is evolutionarily closer to H34. Analysis of the amino acid sequences of gyrA, gyrB, parC and parE genes in M45 and M57 isolates detected that the molecular mechanism of ciprofloxacin resistance is due to the presence of mutational changes in the QRDR of gyrA gene (DNA gyrase subunit A) leading to substitution of serine for leucine at the position 83. No mutations affecting the codons in the QRDR of gyrB, parC and parE genes were detected. Analysis of the gyrA, gyrB, parC and parE genes structure showed a high degree of polymorphism due to the high spontaneous mutations rate. The genomes of M. hominis M45 and M57 isolates found to carry genes of the MATE family efflux pumps, but their role in the development of antimicrobial resistance in M. hominis has not been proved experimentally to date.
Nizhny Novgorod Research Institute of Epidemiology and Microbiology named after I.N. Blokhina, Nizhny Novgorod, Russia
Nizhny Novgorod Research Institute of Epidemiology and Microbiology named after I.N. Blokhina, Nizhny Novgorod, Russia
Nizhny Novgorod Research Institute of Epidemiology and Microbiology named after I.N. Blokhina, Nizhny Novgorod, Russia
Nizhny Novgorod Research Institute of Epidemiology and Microbiology named after I.N. Blokhina, Nizhny Novgorod, Russia
-
1.
Gerasimova N.M., Evstigneeva N.P., Kuznetsova Yu.N. Urogenital infections as an interdisciplinary problem. Modern approaches to diagnosis and treatment. Vestnik poslediplomnogo meditzinskogo obrazovanija. 2009;(1):17-19. Russian.
-
2.
Savicheva A.M. Modern ideas about genital mycoplasms. Mat’ i ditja. 2010;18(4):183-186. Russian.
-
3.
Fofanova I.Yu. Edited by Prilepskaya V.N. Sexually Transmitted Infections. Moscow: GEOTAR-Media, 2014. 157 p. Russian.
-
4.
Rakhmatullina M.R., Kirichenko S.V. Current concepts of genetic variability of genital mycoplasms and their role in the development of inflammatory diseases of the urogenital system. Vestnik dermatologii i venerologii. 2013;(3):17-25. Russian.
-
5.
Chernova O.A., Medvedeva E.S., Muzykantov A.A., Baranova N.B., Chernov V.M. Mycoplasma and their resistance to antibiotics: problems and prospects for controlling mycoplasma infections and contamination of cell cultures. ACTA NATURE. 2016;8 (2Pt29):2738. Russian.
-
6.
Taylor-Robinson D. Infections due to species of Mycoplasma and Ureaplasma: an update. Clin Infect Dis. 2014;18:671-684.
-
7.
Savicheva A.M., Shipitsyna E.V., Bashmakova M.A. Genital mycoplasms – problems of diagnosis and treatment. Clinicheskaja dermatologija i venerologija. 2008;(6):80-90. Russian.
-
8.
Yoshida H., Bogaki M., Nakamura M., Yamanaka L.M., Nakamura S. Quinolone resistance-determining region in the DNA gyrase gyrB gene of Escherichia coli. Antimicrob Agents Chemother. 1991;35(8):1647-1650.
-
9.
Yoshida H., Bogaki M., Nakamura M., Yamanaka L.M., Nakamura S. Quinolone resistance-determining region in the DNA gyrase gyrA gene of Escherichia coli. Antimicrob Agents Chemother. 1990;34(6):1271-1272.
-
10.
Bebear С.М., Renaudin J., Charron A., et al. Mutations in the gyrA, parC, and parE genes associated with fluoroquinolone resistance in clinical isolates of Mycoplasma hominis. Antimicrob Agents Chemother. 1999;43(4):954-956.
-
11.
Gushchin A.E. The role of point mutations in the topoisomerase IV and DNA gyrase genes in developing fluoroquinolone resistance in Mycoplasma hominis. PhD thesis. Moscow, 1999. 102 p. Russian.
-
12.
Govorun V.M. Molecular mechanisms of resistance to tetracyclines and fluoroquinolones in mollikut. PhD thesis. Moscow, 2000. 59 p. Russian.
-
13.
Bebear С.М., Bove J.M., Bebear C., Renaudin J. Characterization of Mycoplasma hominis mutations involved in resistance to fluoroquinolones. Antimicrob Agents Chemother. 1997;41(2):269273.
-
14.
Meng D.Y., Sun C.J., Yu J.B., et al. Molecular mechanism of fluoroquinolones resistance in Mycoplasma hominis clinical isolates. Braz J Microbiol. 2014;45(1):239-242.