SCCmec Typing of Methicillin-Resistant Staphylococcus aureus Strains Isolated from Inpatients and Outpatients in Kazan

Clinical Microbiology and Antimicrobial Chemotherapy. 2013; 15(1):66-71

​ Tyurin Yu.A., Bayazitova L.T., Tyupkina O.F., Fassakhov R.S.
hospital-acquired MRSA communityacquired MRSA SCCmec typing
Section
Personal Experience
Type
Journal article
Publication
Clinical Microbiology and Antimicrobial Chemotherapy. 2013; 15(1):66-71

Objective.

To compare genotypes (by mec complex) of methicillin-resistant S. aureus (MRSA) isolated from different patient populations.

Materials and Methods.

A total of 191 S. aureus strains were included in the study. These strains were isolated during the period of 2007–2011 from patients examined and treated in a specialized outpatient clinic and patients hospitalized to the Republic Teaching Hospital in Kazan. Susceptibility to oxacillin was determined according to NCCLS standards, and MIC90 for oxacillin was determined according to Guidance 4.2.1890- 04 «Antimicrobial susceptibility testing». Genotyping of S. aureus was performed according to the Federal Center of Hygiene and Epidemiology guidelines (2006). SCCmec types were identified by PCR assay using 12 pairs of primers («Sintol», Moscow).

Results.

Of 166 strains isolated from 56 outpatients, 27.1% were identified as MRSA. Of 25 strains isolated from hospitalized patients (trauma unit), 56.0% were identified as MRSA. Genotyping showed that MRSA strains obtained from outpatients harbored SCCmec type IV, and MRSA strains obtained from trauma unit patients harbored SCCmec type II.

Conclusions.

There were found genotypic differences in SCCmec type between MRSA strains from outpatients and MRSA strains from hospitalized patients.

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