A New Method for Evaluation of Antimicrobial Resistance of Bacteria in Biofilms

Clinical Microbiology and Antimicrobial Chemotherapy. 2012; 14(4):303-308

Journal article


At the present article we evaluate a relatively new method of the evaluation of activity of antimicrobials against bacterial biofilms. This method combines the possibilities of simultaneous evaluation of bacterial survival in biofilm in the presence of antimicrobial agent and filtration ability of the biofilm for this antimicrobial agent. The perspective of this method was demonstrated in the model of Staphylococcus aureus biofilm. During the study we evaluated peculiarities of the susceptibility of biofilmforming bacterial cells to pefloxacin. S. aureus survival rates in the biofilm were higher than those for non-biofilm cells. Diffusion rate of pefloxacin through the membrane with biofilm was significantly lower than that through the membrane with non-biofilm forming S. aureus culture.

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