Comparison of Results of Antimicrobial Susceptibility Testing of Gram-negative Aerobic Bacteria by Disc Diffusion on Mueller-Hinton Agar and AGV Medium | CMAC

Comparison of Results of Antimicrobial Susceptibility Testing of Gram-negative Aerobic Bacteria by Disc Diffusion on Mueller-Hinton Agar and AGV Medium

Clinical Microbiology and Antimicrobial Chemotherapy. 2004; 6(2):155-167

​ Stetsiouk O.U., Reshedko G.K.
susceptibility testing disc diffusion media Enterobacteriaceae Pseudomonas aeruginosa
Section
Laboratory Diagnosis
Type
Journal article
Publication
Clinical Microbiology and Antimicrobial Chemotherapy. 2004; 6(2):155-167

Abstract

Results of the comparative susceptibility testing of Gram-negative aerobic bacteria by disc diffusion method on Mueller-Hinton Agar (MHA) and the AGV medium that are widely used for susceptibility testing in Russia, are presented. The aim of the study was to assess the possibility of use of AGV instead of MHA for susceptibility testing by disc diffusion. AGV was shown not to comply with WHO requirements for susceptibility testing media by several key parameters (Ca2+, Mg2+, and thymidine concentrations that are >100, >40 and >20 mg/L, respectively, whereas WHO recommends <50, <25 and <0,03 mg/L, respectively). Inconsistency of AGV composition to WHO requirements for susceptibility testing media, lack of the updated Russian recommendations for susceptibility testing and significant discrepancies of the results obtained on AGV and MHA make it impossible to use AGV for susceptibility testing of Pseudomonas aeruginosa to imipenem, aminoglycosides, and fluoroquinolones, as well as of Enterobacteriaceae to trimethoprim/sulfamethoxazole.

  • 1. National Committee for Clinical Laboratory Standards. Performance standards for antimicrobial susceptibility testing; eleventh informational supplement. 2001: 21 (1).
  • 2. World Health Organization. Manual on antimicrobial resistance and susceptibility testing. Geneva: World Health Organization; 1997.
  • 3. Paladino J.A., Zimmer G.S., Schentag J.J. The economic potential of dual individualization methodologies. Pharmacoeconomics 1996; 10: 539-45.
  • 4. Houang E.T., Hince C., Howard A.J. The effect of composition of culture media on MIC values of antibiotics. In: Russel A.D., Quesnel L.B., editors. Antibiotics: assessment of antimicrobial activity and resistance. The Society of Applied Bacteriology Technical series No. 18. London: Academic Press; 1983. p. 31-48.
  • 5. Bauer A.W., Kirby W.M.M., Sherris J.C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 1966; 45: 493-6.
  • 6. D’Amato R.F., Thornsberry C., Baker C.N. Effect of calcium and magnesium ions on the susceptibility of Pseudomonas species to tetracyclin, gentamicin, polymixin B and carbenicillin. Antimicrob Agents Chemother 1975; 7: 596-600.
  • 7. Чайковская С.М., Навашин С.М., Точеная Н.П. Изменения чувствительности микроорганизмов к антибиотикам под влиянием ионов двухвалентных металлов. Антибиотики 1978; (2):118-22.
  • 8. Bushby S.R.M. Trimethoprim-Sulfamethoxazole: in vitro microbiological aspects. J Infect Dis 1973; 128:S442-S462.
  • 9. Cooper K.E. The theory of antibiotic inhibition zones. In: Kavanagh F., editor. Analytical microbiology. New York: Academic Press; 1964. p. 1-86.
  • 10. Vandepitte J., Engbaek K., Piot P., Heuck C.C. Basic laboratory procedures in clinical bacteriology. Geneva: World Health Organization; 1991.
  • 11. Методические указания по определению чувствительности микроорганизмов к антибиотикам методом диффузии в агарс использованием дисков. М.: Министерство здравоохранения СССР; 1983.
  • 12. Mueller J.H., Hinton J. A protein-free medium for isolation of Gonococcus and Meningococcus. Proceedings of the Society for Experimental Biology and Medicine. 1941; 48:330-3.
  • 13. Altman D.G. Some common problems in medical research. In: Altman D.G., editor. Practical statistics in medical research. London: Chapman & Hal; 1991. p. 396-439.
  • 14. Jorgensen J.H. Selection criteria for antimicrobial susceptibility testing system. J Clin Microbiol 1993; 31: 2841-4.
  • 15. Elder B.L. Verification and validation of procedures in the clinical microbiology laboratory. Clin Microbiol Newsletter 1997; 19:153-6.
  • 16. National Committee for Clinical Laboratory Standards. Development of in vitro susceptibility testing criteria and quality control parameters. Approved guideline. NCCLS Document M23-A. 1994:14(9).
  • 17. Ferone R., Bushby S.R.M., Burchall J.J. Identification of Harper-Crawston factor as thymidine phosphorylase and removal from media of substances interfering with susceptibility testing to sulfonamides and diamino pyrimidines. Antimicrob Agents Chemother 1975; 7:91-8.
  • 18. Koch A.E., Burchal J.J. Reversal of antimicrobial activity of trimethoprim by thymidine in commercially prepared media. Appl Microbiol 1971; 22:812-7.
  • 19. Stokes A., Lacey R.W. Effect of thymidine on activity of trimethoprim and sulfamethoxazole. J Clin Pathol 1978; 31:165-71.
  • 20. Bauer A.W., Sherris J.C. The determination of sulfonamide susceptibility of bacteria. Chemotherapia 1964; 9:1-19.
  • 21. Harper G.J., Crawston W.C. In vitro determination of sulfonamide sensitivity of bacteria. J Pathol Bacteriol 1945; 57:59-66.
  • 22. Jones C. Effect of minimal amounts of thymidine on activity of trimethoprim/sulfamethoxazole against Staphylococcus epidermidis. Antimicrob Agents Chemother 1987; 31:144-7.
  • 23. Then R., Angehrn P. Nature of the bactericidal action of sulfonamides and trimethoprim, alone and in combination. J Infect Dis 1973; 128:498-501.
  • 24. Garrod L.P., Waterwoth P.M. The effect of medium composition on the apparent sensitivity of Pseudomonas aeruginosa to gentamicin. J Clin Pathol 1969; 22:534.
  • 25. Technical recommendations for in vitro susceptibility testing. Report of the Comite de l’Antibiogramme de la Societe Francaise de Microbiologie. Clin Microbiol Infect 1996; 2:S11-S25.
  • 26. Atlas R.M., Parks L.C. Handbook of microbiological media. 2nd ed. Boca Raton: CRC Press; 1997.
  • 27. Blaser J., Dudley M.N., Gilbert D., Inner S.H. Influence of medium and method on the in vitro susceptibility of Pseudomonas aeruginosa and other bacteria to ciprofloxacin and enoxacin. Antimicrob Agents Chemother 1986; 29:927-29.
  • 28. Blaser J., Luthy R. Comparative study of antagonistic effects of low pH and cation supplementation on in vitro activity of quinolones and aminoglycosides against Pseudomonas aeruginosa. J Antimicrob Chemother 1988; 22:15-22.
  • 29. Sabath L.D. Antagonism of antimicrobial agents by products of inflammation. In: Sabath L.D., editor. Action of antibiotics in patients. Bern: Hans Huber Publishers; 1982. p. 74-82.
  • 30. Smith J.T., Ratcliffe N.T. Activity of 4-fluoroquinolone antibacterials at physiological pH values. In: Recent advances in chemotherapy, Antimicrobial section 2: Proceedings of the 14th International Congress of Chemotherapy, 1985. Kyoto, Japan. 1985. p. 1861-2.
  • 31. Birkenhead D., Hawkey P.M., Taylor M.J. The effect of ionic Zn (Zn++) and Nickel (Ni++) on susceptibility of Stenotrophomonas maltophilia to carbapenems. Proceedings of the 35th International Conference on Antimicrobial Agents and Chemotherapy, 1995. San Francisco, USA, 1995. Abstract D 21.
  • 32. Konig C., Simmen H.P., Blaser J. Effect of pathological changes of pH, pO2 and pCO2 on the activity of antimicrobial agents in vitro. Eur J Clin Microbiol Infect Dis 1993; 7:519-26.
  • 33. World Health Organization Expert Committee on Biological Standartization. WHO Technical Report Series 610. Annex 5. Geneva: World Health Organization; 1977. p. 144-78.
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