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The options for the use of «Plot By» tab in the web platform AMRmap are reviewed in the article. Description of some functionality is supported by the specific examples, and Klebsiella pneumoniae was selected as a study object. The analysis elements …

Objective. To study the colonization activity of clinical strains of uropathogenic Escherichia coli (UPEC) against various types of urological catheters. Materials and Methods. Biofilms of E. coli (n = 6) were formed for 24 h according to the standard method. Urological catheters made from latex, polyvinylchloride, silicon and silver-coated silicon were used as a surface for bacterial colonization. The surface roughness and hydrophobicity, biofilm mass, number of colony forming units in biofilm, and frequency of conjugation were assessed.

Objective. To study genetic diversity of Klebsiella pneumoniae strains obtained in the National Medical and Surgical Center named after N.I. Pirogov (Moscow) using next-generation sequencing. Materials and Methods. A total of 19 isolates of K. pneumoniae were included in the study (18 – from patients and 1 – from nosocomial environment). The strains were isolated from January 30 to October 9, 2017 and phenotypically showed multi-drug resistance. DNA sequencing of the strains was performed on the Illumina HiSeq1500 instrument using the Illumina HiSeq PE Rapid Cluster Kit v2 and Illumina HiSeq Rapid SBS Kit v2 kits.

Objective. To evaluate a level of physicians’ awareness of the specific issues of epidemiology, outcomes, prevention and treatment of chronic hepatitis B and C. Materials and Methods. The anonymous questioning was performed among 120 physicians (record of service in a given specialty ranged from 8.1 to 18.6 years) in Kirov city and region. The participants were represented by the following specialties: pediatricians – 41.7% (n=50), obstetricians/gynecologists – 35.8% (n=43), and dentists – 22.

Objective. To study the etiological role and antimicrobial susceptibility of Pseudomonas aeruginosa in one tertiary care hospital with analysis of the influence of antimicrobial stewardship implementation. Materials and Methods. In 2013 primary microbiological evaluation was performed. In 2014 antimicrobial stewardship program was implemented. The impact of the introduction of microbiological monitoring system and antimicrobial stewardship program was evaluated. Results. In total, 461 P. aeruginosa strains were isolated during the study period.

Objective. To evaluate the results of accelerated identification of microorganisms isolated from positive hemocultures obtained from patients of various departments of a large multi-profile hospital by MALDI-TOF MS and to compare them with classical cultural methods. Materials and Methods. In the period from 2017 to 2018, the study included 109 positive blood cultures from 73 patients from UIA GKB number 40 at the age of 1 to 83 years. Blood samples were taken in aseptic conditions from 2 peripheral veins according to the standard protocol in 12 departments of the health facility.

The article presents data on susceptibility to antiseptic preparations in antibiotic-resistant S. aureus and P. aeruginosa strains isolated from burn wounds, which were tested in single-species and double-species biofilms with varying degrees …

Objective. To study level and structure of microbial colonization in cancer patients before admission to a surgery department using molecular methods. Materials and Methods. The presence of DNA of methicillin-resistant Staphylococcus aureus, methicillinresistant coagulase-negative staphylococci, Candida albicans/C. glabrata/C. krusei, Acinetobacter baumannii in the clinical specimens and the presence of antimicrobial resistance genes in cultures were detected by multiplex real-time PCR. A total of 741 clinical samples and 313 cultures were studied.

Objective. To determine the detection rates of HHV-6A and HHV-6B compared to other herpes viruses in children of different age groups. Materials and Methods. A total of 128 patients with symptoms of respiratory viral infections (RVI) aged from 1 to 16 years and 101 otherwise healthy children. A comprehensive examination of the patients, including ELISA, indirect immunofluorescence assay, rapid culture method, PCR, and determination of nucleotide sequences of HHV-6 was performed.

Incidence, severity, and risk factors for hemorrhagic cystitis (HC) were assessed in 267 patients undergoing allogeneic hematopoietic stem cell transplantation (allo-HSCT). HC was diagnosed in 14.6% (39 patients) within 1-139 days after allo-HSCT …