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Personal Experience

Antimicrobial susceptibility testing by broth microdilution method: widely available modification

Objective. To assess the possibility of standard disk application as an antibiotic source for determination of the minimal inhibitory concentrations (MICs) by serial microdilution method. Materials and Methods. A simple method of working solutions preparation using standard paper discs as an antibiotics source available in most microbiological laboratories is proposed. A multiple determination of antibiotic MICs for E. coli ATCC 25922 and P. aeruginosa ATCC 27853 strains was conducted.

Clinical significance and changes in antimicrobial resistance in ESBL producers isolated over the 2014-2016 in a regional teaching hospital

The role of Escherichia coli and Klebsiella pneumoniae in the etiology of respiratory tract, bloodstream, urinary tract and soft tissue infections in the Tyumen Regional Clinical Hospital has been evaluated for three years time period (2014-2016). …

Infectious endocarditis caused by Gemella haemolysans: clinical issues and treatment approaches

Gemella haemolysans is a commensal of the human oral cavity, upper respiratory tract, and gastrointestinal tract, which in some cases can cause serious infections, including infective endocarditis (IE). IE caused by G. haemolysans is more common in …

Use of the MALDI Biotyper system and the microbiological diagnosis algorithm for identification of non-fermenting bacteria isolated from respiratory tract in cystic fibrosis patients

The objective of this study was to compare the MALDI Biotyper system and the microbiological diagnosis algorithm for identification of non-fermenting bacteria isolated from respiratory tract in cystic fibrosis patients. The identification and typing …

Changes in antimicrobial resistance in clinical pediatric isolates of Haemophilus influenzae, Streptococcus pneumoniae, and Moraxella catarrhalis in Middle Ural area

Objective. To reveal the prevalence of antimicrobial resistance of H. influenzae, S. pneumoniae, and M. catarrhalis clinical pediatric isolates in Yekaterinburg and Sverdlovsk region during 2013-2015. Materials and Methods. In total 231 H. influenzae, 289 S. pneumoniae, and 266 M. catarrhalis isolates were included in the study. Antimicrobial susceptibility testing was performed partially by disc-diffusion method and partially by automated method, depending on the local practice; β-lacamase production was detected by the nitrocefin disc test.

The prevalence of parenteral hepatitis markers among the medical personnel in the Russian Federation regions with different intensity of epidemic process

The results of hepatitis markers prevalence estimation and the immune response intensity and duration in hospital staff (Buryatiya, Ulan-Ude, n=611 and Moscow, n=653) after vaccination against hepatitis B are presented. HBV serological markers were …

The rating of medical errors in the use of antimicrobials: retrospective clinical pharmacological analysis

Examination of rational pharmacotherapy is one of the major functional duties of the doctor – the clinical pharmacologist. Chemotherapy as etiotropic therapy is one of the most important types of pharmacotherapy. A retrospective analysis of case …

Pharmacoepidemiology of antimicrobial agents in pregnant women: a non-interventional retrospective study

The present study was conducted within the framework of Saint Petersburg’s multi-purpose programmes initiated in 2014 to restrain antimicrobial resistance. The study was observational and retrospective. The analysis included three maternity …

Compliance of paediatricians to the guidelines on the use of antimicrobials in respiratory infections

Objective. To monitor the level of compliance of paediatricians regarding antimicrobial therapy of acute respiratory infections in children. Materials and Methods. Аnonymous survey of paediatricians of Rostov region was conducted using a questionnaire developed by the authors during 2009-10 and 2014-15. The analysis was performed to assess the compliance with the national recommendations developed by the Union of paediatricians of Russia among the 3 groups of doctors (practicing ≤5 years; 6-20 years; ≥21 years).

Real-Time PCR for the Diagnosis of Tick-Born Infections

Overall 842 cases of admission due to tick bites in Irkutsk area during 2014 were analyzed. Detection of DNA/RNA of the tick-born encephalitis virus, Lyme borreliosis, monocytic ehrlichiosisand granulocyticanaplasmosis in the human serum and in the …