antimicrobial resistance

Objective. To assess the possibility of standard disk application as an antibiotic source for determination of the minimal inhibitory concentrations (MICs) by serial microdilution method. Materials and Methods. A simple method of working solutions preparation using standard paper discs as an antibiotics source available in most microbiological laboratories is proposed. A multiple determination of antibiotic MICs for E. coli ATCC 25922 and P. aeruginosa ATCC 27853 strains was conducted.

Objective. To detect vancomycin-resistant enterococci (VRE) using chromogenic selective medium CHROMagar™VRE (CHROMagar, France). Materials and Methods. In the first part of the study, a total of 39 vancomycin-resistant and 20 vancomycinsusceptible Enterococcus spp. isolated from blood culture with known susceptibility profiles were incubated on the CHROMagar™VRE (CHROMagar, France) and examined after 24 h and 48 h of incubation. In the second part of the study, a total of 110 rectal swabs were taken from patients with hematological malignancies and incubated on the CHROMagar™VRE.

Objective. To study the antimicrobial resistance of H. pylori to clarithromycin, amoxicillin, metronidazole, levofloxacin, tetracycline in adult patients in the Smolensk region. Materials and Methods. Overall, 573 adult patients were included in 2015-2017 and 210 – in 20092010 with positive rapid urease test at the time of gastroscopy for dyspeptic complaints. Antimicrobial susceptibility testing of H. pylori isolates to clarithromycin, amoxicillin, metronidazole, levofloxacin and tetracycline was performed by the agar dilution method.

The role of Escherichia coli and Klebsiella pneumoniae in the etiology of respiratory tract, bloodstream, urinary tract and soft tissue infections in the Tyumen Regional Clinical Hospital has been evaluated for three years time period (2014-2016). …

Objective. Objectives. To research the qualitative relationships between the level of antimicrobial resistance and the level of consumption of different antimicrobial agents, and to further apply this knowledge to minimize the level of resistance. Materials and Methods. An extensive simulation was performed based on the previously implemented model describing the relationship between a resistance and levels of antibiotic consumption. It makes possible to estimate the resistance growth rates in different antibiotic consumption strategies.

Objective. To reveal the prevalence of antimicrobial resistance of H. influenzae, S. pneumoniae, and M. catarrhalis clinical pediatric isolates in Yekaterinburg and Sverdlovsk region during 2013-2015. Materials and Methods. In total 231 H. influenzae, 289 S. pneumoniae, and 266 M. catarrhalis isolates were included in the study. Antimicrobial susceptibility testing was performed partially by disc-diffusion method and partially by automated method, depending on the local practice; β-lacamase production was detected by the nitrocefin disc test.

Objective. To assess regional differences in antimicrobial susceptibility of N. gonorrhoeae in the Russian Federation. Materials and Methods. A total of 365 N. gonorrhoeae strains were isolated over the period of 2015-2016 in Arkhangelsk, Astrakhan, Bryansk, Kaluga, Novosibirsk, Omsk, Ryazan, Tomsk regions, Republic of Tatarstan and Chuvashiya Republic. Antimicrobial susceptibility testing was performed by agar dilution method and assessed according to MUK 4.2.1890-04 criteria using WHONET software (v5.4). Molecular typing was performed according to the NG-MAST protocol.

After the introduction of the conjugated 7-valent pneumococcal vaccine (PCV7) the incidence of infections, caused by non-vaccine serotypes of S. pneumoniae, especially by 19А serotype, significantly increased. But in few countries similar phenomenon …

The study included 418 of the 841 clinical isolates of S. aureus, which were regarded as nosocomial. These 418 clinical nosocomial S. aureus strains were isolated in 2013-2014 from patients hospitalized in 25 hospitals in 19 Russian cities. …

Species of the family Enterobacteriaceae represent the most prevalent group of nosocomial pathogens. In this paper, we report the data on antimicrobial susceptibility of 1670 isolates of Enterobacteriaceae collected in 35 hospitals of 22 cities of …