antimicrobial resistance

Experience with the AMRcloud online platform for antimicrobial resistance surveillance in zoonotic bacteria

Objective. To develop an approach to comprehensive analysis and presentation of data on antibiotic resistance of zoonotic bacteria to minimize antibiotic resistance in the medical and veterinary fields using the AMRcloud platform. Materials and Methods. Isolates of E. coli, Salmonella spp., Enterococcus spp. and Campylobacter spp. were taken from farm animals, food and feed. AST was performed by broth microdilution method. Genes of resistance were detected by whole-genome sequencing or PCR.

Antimicrobial resistance of clinical Streptococcus pyogenes isolates in Russia: the results of multicenter epidemiological study «PEHASus 2014–2017»

Objective. To evaluate antimicrobial resistance patterns of clinical Streptococcus pyogenes isolates from different regions of Russia during 2014–2017. Materials and Methods. A total of 792 clinical S. pyogenes isolates from 14 Russian cities were included in the study. Susceptibility testing was performed using reference broth microdilution method (ISO 207761:2006). Susceptibility testing results were interpreted using EUCAST v.10.0 breakpoints. Results. Penicillin G was active against all tested isolates with the MIC50–90 values of 0.

The reasons why Klebsiella pneumoniae becomes a leading opportunistic pathogen

This review provides an analysis of causes why Klebsiella pneumoniae takes a leading place among opportunistic human bacteria. The review includes the history of K. pneumoniae studies, microbiological properties and various Klebsiella-associated …

Antimicrobial resistance of Haemophilus influenzae clinical isolates in Russia: the results of multicenter epidemiological study «PEHASus 2014–2017»

Objective. To evaluate antimicrobial resistance patterns of clinical Haemophilus influenzae isolates from different regions of Russia during 2014–2017. Materials and Methods. We included in the study 185 clinical H. influenzae isolates from 13 Russian cities. Susceptibility testing was performed by reference broth microdilution method (ISO 207761:2006). Susceptibility testing results were interpreted using EUCAST v. 10.0 breakpoints. Results. H. influenzae isolates were highly susceptible to all (96.8%-100%) β-lactams tested (amoxicillin/ clavulanate, cefixime, ceftaroline, ceftibuten, cefditoren, ertapenem) with the exception of ampicillin (15.

To stop the progress of antimicrobial resistance today means to give a chance of mankind surviving tomorrow

Antimicrobial resistance is a global challenge for human society. Scientists around the world are trying not to return to the time when antimicrobials were not yet discovered, and millions of people were dying from infections. This article describes …

Antimicrobial resistance of clinical Streptococcus pneumoniae isolates in Russia: the results of multicenter epidemiological study «PEHASus 2014–2017»

Objective. To evaluate antimicrobial resistance patterns of clinical Streptococcus pneumoniae isolates from different regions of Russia during 2014–2017. Materials and Methods. We included in the study 519 clinical S. pneumoniae isolates from 18 Russian cities. Susceptibility testing was performed by reference broth microdilution method (ISO 20776-1:2006). Susceptibility testing results were interpreted according to EUCAST v.9.0 breakpoints. Results. Using EUCAST non-meningeal interpretation criteria, 65.1% of isolates tested were susceptible (S) to penicillin; in addition 28.

Cefotaxime/sulbactam: clinical and pharmacological characteristics

The recently approved cefotaxime/sulbactam, combination of third-generation cephalosporin (cefotaxime) and beta-lactamase inhibitor (sulbactam), can combat resistance mechanisms associated with the production of serine beta-lactamases, including some …

Antimicrobial susceptibility of Streptococcus pneumoniae in Kazakhstan

Objective. To study antimicrobial susceptibility of Streptococcus pneumoniae clinical isolates obtained from the patients with community-acquired pneumonia in the Central Kazakhstan. Materials and Methods. A total of 186 clinical strains of S. pneumoniae were obtained from the patients with respiratory tract infections in the Central Kazakhstan over the period of 2012–2017. Antimicrobial susceptibility was determined by disk-diffusion method. The isolation and identification of pathogens was performed by routine methods. Antimicrobial (oxacillin, vancomycin, clindamycin, azithromycin, chloramphenicol, tetracycline, rifampin, levofloxacin, linezolid, trimethoprim/sulfamethoxazole) susceptibility testing was performed and interpreted according to CLSI criteria (M100-24).

Application of AMRmap: «from the general to the specific» approach by the example of Klebsiella pneumoniae

The options for the use of «Plot By» tab in the web platform AMRmap are reviewed in the article. Description of some functionality is supported by the specific examples, and Klebsiella pneumoniae was selected as a study object. The analysis elements …

Antimicrobial resistance, carbapenemase production, and genotypes of nosocomial Acinetobacter spp. isolates in Russia: results of multicenter epidemiological study ”MARATHON 2015–2016”

Objective. Objectives. To assess the rates of antibiotic resistance and production of acquired carbapenemases in nosocomial strains of Acinetobacter spp., and to determine the genotypes and prevalence of “international high-risk clones” among nosocomial strains of Acinetobacter baumannii isolated in various regions of Russia within the “MARATHON 2015–2016” study. Materials and Methods. A total of 1005 non-duplicate nosocomial isolates of Acinetobacter spp., including 975 isolates of A. baumannii, collected in 44 hospitals from 25 cities in Russia in 2015– 2016 were studied.