Investigation of a candidemia outbreak caused by C. parapsilosis in a pediatric hematology/oncology center | CMAC

Investigation of a candidemia outbreak caused by C. parapsilosis in a pediatric hematology/oncology center

Clinical Microbiology and Antimicrobial Chemotherapy. 2020; 22(4):266-270

​ Kozhushnaya O.S., Solopova G.G., Voropaev A.D., Markova Zh.V., Satsuk A.V., Balamozhnova A.O., Novichkova G.A.
10.36488/cmac.2020.4.266-270
сandidemia Candida parapsilosis immunocompromised children short tandem repeats microsatellite repeats
Section
Diseases and Pathogens
Type
Original Article
Publication
Clinical Microbiology and Antimicrobial Chemotherapy. 2020; 22(4):266-270

Objective.

To investigate a candidemia outbreak caused by C. parapsilosis in a clinical unit of the Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology (NMRC PHOI).

Materials and Methods.

A total of 35 isolates of C. parapsilosis obtained from clinically significant specimens and swabs, including hands of nursing staff of the NMRS PHOI, over the 2018-2020 were genotyped in this retrospective study. Identification of C. parapsilosis isolates was performed by microbiological methods. The clonal structure of C. parapsilosis isolates was investigated by polymerase chain reaction followed by fragment analysis of microsatellite repeats (short tandem repeats, STR markers).

Results.

The results of the study showed genetic diversity of the population of C. parapsilosis isolates over the 2018–2020 in the NMRC PHOI. A total of 27 genotypes were identified, one of which caused candidemia in 6 patients.

Conclusions.

The study results confirmed the nosocomial candidemia outbreak and showed the fragment analysis of STR-markers may be used for epidemiological investigations of outbreaks in hospital settings.

Kozhushnaya O.S.

Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia

Galina G. Solopova

Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia

Voropaev A.D.

Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia

Markova Zh.V.

Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia

Satsuk A.V.

Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia

Balamozhnova A.O.

Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia

Novichkova G.A.

Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia

  • 1. Lamoth F., Lockhart S.R., Berkow E.L., Calandra T. Changes in the epidemiological landscape of invasive candidiasis. J Antimicrob Chemother. 2018;73(suppl_1):i4-i13. DOI: 10.1093/jac/dkx444
  • 2. Ashford B.K. Certain conditions of the gastro-intestinal tract in Porto Rico and their relation to tropical sprue. Am J Trop Med. 1928;8(6):507-538.
  • 3. Joachim H., Polayes S.H. Subacute endocarditis and systemic mycosis (monilia). J Am Med Assoc. 1940;115(3):205. DOI: 10.1001/jama.1940.72810290001009
  • 4. Badali H., Rezaie S., Meis J.F., Agha Kuchak Afshari S., Modiri M., Hagen F., et al. Microsatellite genotyping of Candida parapsilosis clinical Isolates. Curr Med Mycol. 2017;3(4):15-20. DOI: 10.29252/cmm.3.4.15
  • 5. Diab-Elschahawi M., Forstner C., Hagen F., Meis J.F., Lassnig A.M., Presterl E., et al. Microsatellite genotyping clarified conspicuous accumulation of Candida parapsilosis at a cardiothoracic surgery intensive care unit. J Clin Microbiol. 2012;50(11):3422-3426. DOI: 10.1128/JCM.01179-12
  • 6. Sabino R., Sampaio P., Rosado L., Videira Z., Grenouillet F., Pais C. Analysis of clinical and environmental Candida parapsilosis isolates by microsatellite genotyping-a tool for hospital infection surveillance. Clin Microbiol Infect. 2015;21(10):954.e1-954.e8. DOI: 10.1016/j.cmi.2015.06.001
  • 7. Wang H., Zhang L., Kudinha T., Kong F., Ma X.J., Chu Y.Z., et al. Investigation of an unrecognized large-scale outbreak of Candida parapsilosis sensu stricto fungaemia in a tertiary-care hospital in China. Sci Rep. 2016;6:27099. DOI: 10.1038/srep27099
  • 8. Francisco A.P., Bugalho M., Ramirez M., Carriço J.A. Global optimal eBURST analysis of multilocus typing data using a graphic matroid approach. BMC Bioinformatics. 2009;10:152. DOI: 10.1186/1471-2105-10-152
  • 9. Solopova G.G., Pirumova R.P., Guseva G.D., Miakova N.V., Litvinov D.V., Khamin I.G., et al. Candidemias in children with oncohematological diseases: risk factors, etiologic spectrum and therapy results. Pediatrija. 2019;98(1):1827. Russian. DOI: 10.24110/0031-403X-2019-98-1-18-27
  • 10. Singh R., Parija S.C. Candida parapsilosi : an emerging fungal pathogen. Indian J Med Res. 2012;136(4):671673. PMID: 23168709
  • 11. Markova I.V., Rogacheva Yu.A., Popova M.O., Volkova A.G., Ekushov K.A., Frolova A.S., et al. Invasive candidiasis in children after hematopoietic stem cell transplantation. Voprosy gematologii/onkologii i immunopatologii v pediatrii. 2019;18(2):5358. Russian. DOI: 10.24287/1726-17082019-18-2-53-58
  • 12. Saiman L., Ludington E., Pfaller M., Rangel‐Frausto M.S., Wiblin R.T., Dawson J., et al. Risk factors for candidemia in Neonatal Intensive Care Unit patients. Pediatr Infect Dis J. 2000;19(4):319-324. DOI: 10.1097/00006454200004000-00011
  • 13. Bonassoli L.A., Bertoli M., Svidzinski T.I.E. High frequency of Candida parapsilosis on the hands of healthy hosts. J Hosp Infect. 2005;59(2):159-162. DOI: 10.1016/j.jhin.2004.06.033
  • 14. Clark T.A., Slavinski S.A., Morgan J., Lott T., ArthingtonSkaggs B.A., Brandt M.E., et al. Epidemiologic and molecular characterization of an outbreak of Candida parapsilosis bloodstream infections in a community hospital. J Clin Microbiol. 2004;42(10):4468-4472. DOI: 10.1128/JCM.42.10.4468-4472.2004
  • 15. Huang Y.-C., Lin T.-Y., Leu H.-S., Peng H.-L., Wu J.-H., Chang H.-Y. Outbreak of Candida parapsilosis fungemia in neonatal intensive care units: Clinical implications and genotyping analysis. Infection. 1999;27(2):97-102. DOI: 10.1007/BF02560505
  • 16. Levin A.S., Costa S.F., Mussi N.S., Basso M., Sinto S.I., Machado C., et al. Candida parapsilosis Fungemia associated with implantable and semi-implantable central venous catheters and the hands of healthcare workers. Diagn Microbiol Infect Dis. 1998;30(4):243-239. DOI: 10.1016/s0732-8893(98)00006-6
  • 17. Lupetti A., Tavanti A., Davini P., Ghelardi E., Corsini V., Merusi I., et al. Horizontal transmission of Candida parapsilosis candidemia in a neonatal intensive care unit. J Clin Microbiol. 2002;40(7):2363-2369. DOI: 10.1128/jcm.40.7.2363-2369.2002
  • 18. Saxén H., Virtanen M., Carlson P., Hoppu K., Pohjavuori M., Vaara M., et al. Neonatal Candida parapsilosis outbreak with a high case fatality rate. Pediatr Infect Dis J. 1995;14(9):776-781. DOI: 10.1097/00006454199509000-00009
  • 19. van Asbeck E.C., Huang Y.-C., Markham A.N., Clemons K.V., Stevens D.A. Candida parapsilosis fungemia in neonates: genotyping results suggest healthcare workers hands as source, and review of published studies. Mycopathologia. 2007;164(6):287-293. DOI: 10.1007/s11046-007-9054-3
  • 20. Fominykh V.V., Klyasova G.A., Maschan A.A. Epidemic outbreak of candidemia caused by unusual types of Candida non-albicans: clinical course patterns and search for the source. Onkogematologija. 2011;(1):39-44. Russian. DOI: 10.17650/1818-8346-2011-6-1-39-44
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