Clinical Microbiology and Antimicrobial Chemotherapy. 2015; 17(1):73-78
The results of the immune response intensity and duration in 890 hospital staff after vaccination against hepatitis B are presented. High frequency of HBsAg (1,7%), anti-HBs (21,1%), anti-HВe (16,9%) among not vaccinated against hepatitis B medical staff in comparison with vaccinated persons (0,2, 10,7 and 6,4% respectively) was established. «Isolated» anti-HBc was estimated to occur at 5 (0,8%) vaccinated and 15 (6,3%) not vaccinated persons, HBV DNA — at 3 (0,5%) and 5 (2,1%), respectively. Among 653 vaccinated medical staff antiHBs were absent or their concentration was below protective level at 140 (21,4%) persons. HBV DNA positive in PCR isolates had genotype D, subtypes ayw3 and ayw2. Sequence analysis of PCR products amplified from the S region revealed site replacements T118A, T118V, M125T, A128V. The necessity of the new generation vaccines formation capable to protect from HBV «wild» strains and mutant forms is discussed.