Susceptibility of Nosocomial K. pneumoniae, P. aeruginosa, A. baumannii, and P. mirabilis Strains to a Chlorhexidine-Based Antiseptic Preparation

Clinical Microbiology and Antimicrobial Chemotherapy. 2015; 17(1):57-66

​ Detusheva E.V., Rodin V.B., Slukin P.V., Ershova O.N., Aleksandrova I.A., Kurdyumova N.V., Sazykina S.Yu., Dyatlov I.A., Fursova N.K.
chlorhexidine nosocomial resistance healthcare-associated infections planktonic bacteria biofilm
Section
Personal Experience
Type
Journal article
Publication
Clinical Microbiology and Antimicrobial Chemotherapy. 2015; 17(1):57-66

Abstract

This study was performed to investigate activity of chlorhexidine-based antiseptic preparation «Dezin» against the following pathogens causing nosocomial respiratory tract infections (RTI) and urinary tract infections (UTI): Klebsiella pneumoniae (n=8), Pseudomonas aeruginosa (n=3), Acinetobacter baumannii (n=4) and Proteus mirabilis (n=3) isolated from patients in neurosurgery ICU in 2013, as well as reference strains of K. pneumoniae (n=1), P. aeruginosa (n=1), Escherichia coli (n=1), and Staphylococcus aureus (n=1). Antimicrobial activity of the antiseptic preparation was evaluated by microbial growth inhibition in planktonic state (microdrop) and protobiofilm-like state (cellulose patch). Distribution of tested strains by susceptibility of planktonic cells to chlorhexidine (expressed in minimal bactericidal concentration [MBC]) was the following (in order of increasing susceptibility): P. mirabilis (0.025%), P. aeruginosa (0.009%), K. pneumoniae (0.006%), A. baumannii (0.006%), E. coli (0.005%) и S. aureus (0.005%). Susceptibility of biofilmlike associations (on the surface of cellulose patches) to chlorhexidine was significantly decreased: 23 times in P. aeruginosa (MBC – 0.2%), 28 times in A. baumannii (MBC — 0.16%), 48 times in K. pneumoniae (MBC – 0.3%), 50 times in P. mirabilis (MBC — 1.3%), 128 times in E. coli (MBC — 0.6%), and 255 times in S. aureus (MBC — 1.3%). Based on the results, 1.5% chlorhexidine solution may be recommended as a measure to prevent nosocomial RTIs and UTIs in neurosurgery ICU patients.

Elena V. Detusheva

State Research Center for Applied Microbiology and Biotechnology, Obolensk, Russia

Rodin V.B.

State Research Center for Applied Microbiology and Biotechnology, Obolensk, Russia

Slukin P.V.

State Research Center for Applied Microbiology and Biotechnology, Obolensk, Russia

Ershova O.N.

Research Institute of Neurosurgery named after N.N. Burdenko, Moscow, Russia

Aleksandrova I.A.

Research Institute of Neurosurgery named after N.N. Burdenko, Moscow, Russia

Kurdyumova N.V.

Research Institute of Neurosurgery named after N.N. Burdenko, Moscow, Russia

Sazykina S.Yu.

Research Institute of Neurosurgery named after N.N. Burdenko, Moscow, Russia

Dyatlov I.A.

State Research Center for Applied Microbiology and Biotechnology, Obolensk, Russia

Fursova N.K.

State Research Center for Applied Microbiology and Biotechnology, Obolensk, Russia

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