Clinical Microbiology and Antimicrobial Chemotherapy. 2014; 16(3):238-243
Using the results from the previous experiments on N-glycans of hepatitis C virus (HCV) glycoprotein E1 in the process of structure viral proteins assembling in the model system (insect cells Sf9 and baculovirus expression system), simulation of protein E1 structure with different number and positions of glycans is presented in this paper. Computer simulation of spatial pattern of mutant E1 proteins was performed using the method of molecular dynamics and computer processing power. Results of this study demonstrate that N-linked glycans have a direct effect on protein E1 folding and significantly differ in molecule configuration pattern.