Clinical Microbiology and Antimicrobial Chemotherapy. 2007; 9(3):211-218
More frequently metallo-beta-lactamase (MβL) production becomes a reason of resistance to beta-lactam antibiotics among non-fermentative Gram-negative bacteria, foremost among Pseudomonas spp. and Acinetobacter spp. pathogens. It is evident that availability of reliable MβL detection methods in microbiology laboratories is an indispensable condition demanded for effective infection control and rapid spread prevention of this resistance mechanism. In this review we show some MβL detection methods in Gram-negative bacteria. EDTAdouble-disc synergy test is a useful tool for phenotypic screening of MβL, which is based on synergy detection between ceftazidime, imipenem, meropenem and ethylene diamine tetraacetic acid (EDTA) in MβL-producing strains. In addition to it multiplex real-time polymerase chain reaction (PCR) analysis allows to identify internal fragments of two most common MβL-coding genes: VIM and IMP.