Abstract
More frequently metallo-beta-lactamase (MβL) production becomes a reason of resistance to beta-lactam antibiotics among non-fermentative Gram-negative bacteria, foremost among Pseudomonas spp. and Acinetobacter spp. pathogens. It is evident that availability of reliable MβL detection methods in microbiology laboratories is an indispensable condition demanded for effective infection control and rapid spread prevention of this resistance mechanism. In this review we show some MβL detection methods in Gram-negative bacteria. EDTAdouble-disc synergy test is a useful tool for phenotypic screening of MβL, which is based on synergy detection between ceftazidime, imipenem, meropenem and ethylene diamine tetraacetic acid (EDTA) in MβL-producing strains. In addition to it multiplex real-time polymerase chain reaction (PCR) analysis allows to identify internal fragments of two most common MβL-coding genes: VIM and IMP.
-
1.
Walsh T.R., Toleman M.A., Poirel L., Nordmann P. Metallo-beta-lactamases: the quiet before the storm? Clin Microbiol Rev 2005; 18:306-25.
-
2.
Cornaglia G., Akova M., Amicosante G., et al. Metallo-βlactamases as emerging resistance determinants in Gramnegative pathogens: open issues. Int J of Antimicrob Agents 2007; 29:380-8.
-
3.
Bush K., Jacoby G.A., Medeiros A.A. A functional classification scheme for beta-lactamases and its correlation with molecular structure. Antimicrob Agents Chemother 1995; 39:1211-33.
-
4.
Castanheira M., Toleman M.A., Jones R.N., Schmidt F.J., Walsh T.R. Molecular characterization of a betalactamase gene, blaGIM-1, encoding a new subclass of metallo-beta-lactamase. Antimicrob Agents Chemother 2004; 48:4654-61.
-
5.
Lee K., Yum J.H., Yong D., et al. Novel acquired metalloβ-lactamase gene, blaSIM-1, in a class 1 integron from Acinetobacter baumannii clinical isolates from Korea. Antimicrob Agents Chemother 2005; 49:4485-91.
-
6.
Toleman M.A., Simm A.M., Murphy T.A., et al. Molecular characterization of SPM-1, a novel metallo-β-lactamase isolated in Latin America: report from the SENTRY antimicrobial surveillance programme. J Antimicrob Chemother 2002; 50:673-9.
-
7.
Osano E., Arakawa Y., Wacharotayankun R., et al. Molecular characterization of an enterobacterial metallo beta-lactamase found in a clinical isolate of Serratia marcescens that shows imipenem resistance. Antimicrob Agents Chemother 1994; 38:71-8.
-
8.
Arakawa Y., Murakami M., Suzuki K., et al. A novel integron-like element carrying the metallo-beta-lactamase gene blaIMP. Antimicrob Agents Chemother 1995; 39:1612-5.
-
9.
Poirel L., Lambert T., Turkoglu S., et al. Characterization of Class 1 integrons from Pseudomonas aeruginosa that contain the blaVIM-2 carbapenem-hydrolyzing beta-lactamase gene and of two novel aminoglycoside resistance gene cassettes. Antimicrob Agents Chemother 2001; 45:546-52.
-
10.
Определение чувствительности микроорганизмов к антибактериальным препаратам. Методические указания МУК 4.2.1890-04. Государственное санитарно-эпидемиологическое нормирование Российской Федерации. Москва, 2004.
-
11.
Методические рекомендации по проведению работ в диагностических лабораториях, использующих метод полимеразной цепной реакции (№ 19/52-17, утверждены Государственным комитетом санитарноэпидемиологического надзора РФ 22 июня 1995 г.). Москва, 1995.