Personal Experience

Objective. To study antimicrobial effect of extracellular metabolites of probiotic L. plantarum 8P-A3 strain on antibiotic-resistant Klebsiella pneumoniae and Acinetobacter baumannii isolated from infected wounds. Materials and Methods. Bacteriological methods: semiquantitative sector method for extracting opportunistic microorganisms from an infected wound; a method for the identification of the antimicrobial activity of metabolites in a liquid milieu; The identification of bacteria was implemented with the help of the MALDI-TOF Autoflex mass spectrometer (Bruker Daltonics, Germany).

Objective. To analyze and compare multidrug-resistant Klebsiella pneumoniae (KP) strains isolated from different sites of the same patients. Materials and Methods. A total of 30 KP strains isolated from 15 patients treated in intensive care and surgical units in 2020–2021 were studied. Two strains isolated from one child were selected for analyses. One strain was isolated from a clinically significant site (blood/urine/wound/endotracheal aspirate or sputum), the other from a monitoring site (stool).

Objective. To evaluate the ability of bovhyaluronidaze azoximer to destroy biofilms formed by clinical strains of microorganisms. Materials and Methods. The study included 50 clinical strains of Staphylococcus aureus (n = 25), Staphylococcus epidermidis (n = 6), Enterococcus faecalis (n = 8), Escherichia coli (n = 9), Candida albicans (n = 2). Antimicrobial susceptibility testing was performed using broth microdilution method. Biofilm formation culturing in presence of antibiotics, or bovhyaluronidaze azoximer, or their combinations was assessed in Mueller-Hinton broth and brain heart broth in 96-well plates.

Objective. To study the population and genetic structure of S. pneumoniae isolated from children with cystic fibrosis (CF) and congenital malformations of the bronchi and lungs (CM) over the period 2011 to 2021, their serotype diversity in pre-vaccination and post-vaccination periods and susceptibility to antimicrobials. Materials and Methods. A total of 140 S. pneumoniae isolates (during 2011-2021) from respiratory samples of patients with CM or CF were included in the study.

Objective. To develop a technology for producing Russia-made Mueller-Hinton broth and to compare its quality in terms of physicochemical and microbiological parameters with a foreign analog in trials using reference microbial strains sharing common and complex nutritional requirements. Materials and Methods. The work investigated casein acid hydrolysates and Mueller-Hinton broths (MHBs) produced by the Federal Budgetary Institution of Science SRCAMB (MHB–Obolensk) and by BD BBL (MHB–BD) through testing 7 test strains of E.

Objective. To evaluate the dynamics of antimicrobial resistance of Escherichia coli isolates from the urine of patients hospitalized in medical institutions of Tashkent city from 2018 to 2021. Materials and Methods. The study included 1020 E. coli isolates from the urine of patients with clinical symptoms of urinary tract infections admitted to the reference laboratory of the Antimicrobial Resistance Research Center (ARRC) from three medical institutions in Tashkent city for the period 2018– 2021.

Objective. To identify specific features of the clinical course of Congo-Crimean hemorrhagic fever (CCHF) in children in order to optimize early and differential diagnosis, management tactics and treatment of pediatric patients Materials and Methods. A retrospective analysis of 159 probable and confirmed cases of Congo-Crimean hemorrhagic fever was carried out in patients admitted to the infectious diseases departments of Shymkent, district hospitals of Turkestan region for the period 2013–2022, of which 39 (24.

Objective. To determine the level of knowledge of doctors on the rational use of antimicrobial drugs in clinical practice and to compare with data of the previous stage of the study and results obtained from students in a parallel project. Materials and Methods. A multicenter questionnaire survey of practitioners with analysis of results – stage of KANT project (full name of the project – «Physicians’ (Students’) knowledge in antimicrobials usage») conducted in 2020–2023.

Objective. The aim of the study was to develop gradient diffusion strips with substances capable of potentiating the action of antibiotics for the treatment of infections caused by multidrug-resistant strains of K. pneumoniae. Materials and Methods. The substances azidothymidine and baicalin were used to evaluate the joint action of combinations of antibiotics with substances that potentiate their action. Determination of susceptibility to combinations of antibiotics (gentamicin, cefotaxime, ciprofloxacin and chloramphenicol) with the listed substances was carried out by a modified gradient-diffusion method (cross-test).

Objective. To determine the level of basic knowledge of 5–6-year medical students in the issues of appropriate antimicrobial therapy. Materials and Methods. A multicenter questionnaire survey with analysis of results – stage of KANT project (full name of the project – «Physicians’ (Students’) knowledge in antimicrobials usage») conducted in 2020-2023. Results. A total of 277 students in 6 regions of Russia were surveyed. The average level of correct answers for the whole questionnaire amounted to 42.