Personal experience

The efficacy and safety of direct-acting antiviral agents in patients with chronic HCV infection and UGT1A1*28 polymorphism

Objective. To determine the efficacy and safety of direct-acting antiviral agents (DAA) in patients with chronic HCV infection and UGT1A1*28 polymorphism. Materials and Methods. An open-label, non-randomized, observational study to assess efficacy and safety of DAA in patients (n = 143) with chronic hepatitis C (CHC) and liver cirrhosis and UGT1A1*28 polymorphism was performed. A total of 139 patients with chronic HCV infection were included in the efficacy analysis (absence of HCV RNA in blood by PCR) by the rate of sustained virologic response at week 12 (SVR12).

The effect of the surgical hemostatic product «Hemoblock»TM on in vitro bacterial colonization

Objective. To evaluate effect of «Haemoblock»TM preparation on in vitro bacterial colonization. Materials and Methods. Activity of «Haemoblock»ТМ product on the growth and colonization of S. aureus АТСС 25923, S. epidermidis ATCC 28922, E. coli АТСС 25922, P. aeruginosa АТСС 27853 cells in the suspension and on the surface of monofilament macroporous polyester mesh was evaluated. Results. This product at concentration of 0.062% and above inhibited bacterial growth and reduced biofilm mass and bacterial cell viability.

Therapeutic drug monitoring of vancomycin in patients with infectious complications in traumatology and orthopedics

Objective. The purpose of this study was to study the frequency of achievement of target values of vancomycin residual concentration (RC) and to identify the factors influencing this indicator. Materials and Methods. A retrospective observational study of vancomycin RC was performed in 471 patients who received vancomycin infusions in the department of purulent surgery of the RSRI of TO named after R.R. Vreden about capillary instillation after major orthopedic operations over the period from 01.

Successful treatment of infective endocarditis caused by Listeria monocytogenes in a patient after previous open-heart surgery (clinical case)

Listeriosis has a variety of clinical manifestations from febrile gastroenteritis to bacteremia, sepsis, meningoencephalitis and infective endocarditis with valvular heart disease. In this report, we present a rare case of successful treatment of …

The effect of the tinctorial properties of microorganism on the nutritional status and the early outcome in patients with SIRS of bacterial origin

Objective. To determine the effect of the tinctorial properties of microorganism on the nutritional status and the early outcome in patients with systemic inflammatory response syndrome (SIRS) of bacterial origin. Materials and Methods. A retrospective statistical analysis of case histories of 137 patients with signs and symptoms of SIRS and bacterial infections (caused by a single pathogen) was performed. The following clinical and laboratory parameters of protein-energy undernutrition (PEU) were determined and assessed: body mass index, body weight deficit, total protein, serum albumin, absolute peripheral blood lymphocyte count.

Mutations in the cytomegalovirus UL97 gene associated with ganciclovir resistance in recipients of allogeneic hematopoietic stem cell transplants

Objective. To identify mutations in UL97 gene associated with antiviral drug resistance in recipients of allogeneic hematopoietic stem cells transplants (allo-HSCT). Materials and Methods. A total of 9 HCMV DNA samples were studied. These samples were received from the blood of 8 allo-HSCT recipients who were infected with HCMV and undergoing treatment at NMRC of Hematology (Russia) over the period of 2016 to 2017. Sanger sequencing was used to find mutations.

Epidemiological significance of genome variations in Pseudomonas aeruginosa causing chronic lung infection in patients with cystic fibrosis

Objective. To present the data on the main mechanism of molecular variation in P. aeruginosa causing chronic lung infection in patients with cystic fibrosis. Materials and Methods. A total of 1800 throat swabs and sputum samples from cystic fibrosis patients were included in the study over the 10-year period. P. aeruginosa isolates were primarily identified by the biochemical method using the API 20NE test strips (bioMerieux, France). Antimicrobial susceptibility testing was performed by disc diffusion method.

Clinical case of difficult differential diagnosis between liver cirrhosis and hepatocellular carcinoma associated with HCV infection in a patient with prolonged alcohol exposure

This paper presents a clinical case of large focal liver mass in a 55-year old patient with HCV-associated liver cirrhosis and prolonged high-dose alcohol exposure. Given the inconsistency between clinical data and ultrasound examination results as …

Integrated analysis of antibiotic consumption in the hospital segment of Saint-Petersburg in 2014–2018

Objective. To estimate composite indicators of systemic antibiotic consumption in the hospital segment of Saint-Petersburg in 2014–2018. Materials and Methods. Information on the volume and structure of AMP consumption in the hospital segment of St. Petersburg in 2014–2018 was obtained from AlfaRm Databases. The amount of purchased AMP was recalculated in the number of average daily maintenance doses (Defined Daily Dose, DDDs), as well as DDDs per 100 bed-days (DDDh) for each international nonproprietary name of AMP.

Antimicrobial susceptibility of Streptococcus pneumoniae in Kazakhstan

Objective. To study antimicrobial susceptibility of Streptococcus pneumoniae clinical isolates obtained from the patients with community-acquired pneumonia in the Central Kazakhstan. Materials and Methods. A total of 186 clinical strains of S. pneumoniae were obtained from the patients with respiratory tract infections in the Central Kazakhstan over the period of 2012–2017. Antimicrobial susceptibility was determined by disk-diffusion method. The isolation and identification of pathogens was performed by routine methods. Antimicrobial (oxacillin, vancomycin, clindamycin, azithromycin, chloramphenicol, tetracycline, rifampin, levofloxacin, linezolid, trimethoprim/sulfamethoxazole) susceptibility testing was performed and interpreted according to CLSI criteria (M100-24).