Personal Experience

Objective. To study the characteristics of microbial landscape in patients with chronic obstructive pulmonary disease and chronic simple bronchitis of occupational etiology from mineral dust exposure (COPD OE) with different clinical phenotypes. Materials and Methods. Patients were divided into 3 groups. Group-1 – 10 patients with COPD OE, clinical phenotype “B” (GOLD II); Group-2 – 19 patients with COPD OE, clinical phenotype “E” (GOLD III-IV); Group-3 – 11 patients with chronic simple bronchitis (CSBPE).

Objective. To analyze antimicrobial susceptibility of representatives of the genus Streptococcus in different wards of a multidisciplinary hospital. Materials and Methods. Epidemiological observational descriptive continuous retrospective survey was conducted. Antimicrobial susceptibility data on 2916 streptococcal isolates obtained from the laboratory information system «ALISA» were analyzed for the period from 01.01.2017 to 31.12.2022. Antimicrobial susceptibility testing was performed using BD Phoenix M50, interpretation of susceptibility testing results was done in accordance with EUCAST recommendations.

Objective. To study the microbial composition of biopsies in inflammatory bowel diseases. Materials and Methods. From November 2019 to February 2023, 100 patients of the coloproctology department with inflammatory bowel diseases (IBD) were examined: 65 patients with ulcerative colitis and 35 patients with Crohn’s disease. A metagenomic analysis of biopsies (DNA sequencer Illumina, USA) obtained during endoscopic examination using a standard technique, with a bioiformatic data analysis, was performed. The diversity of microbiomes in the samples was assessed using the Fisher index.

Objective. To assess the ability of clinical isolates of opportunistic microorganisms to accumulate biofilm biomass under the influence of composite disinfectants. Materials and Methods. The study included 53 strains (49 – K. pneumoniae, 4 – P. aeruginosa) obtained from two multidisciplinary children’s hospitals at the city and regional levels. Specimens were collected from different loci of patients, medical devices, and environmental objects. To assess the reaction of biofilms (BF) of clinical isolates to the effects of disinfectants (DIs), three composite DIs were used: No.

Objective. To perform comparative analysis of Bordetella protein profiling data and their dependence on various nutrient media. Materials and Methods. This prospective open single-center study, the protein profiles of B. pertussis, B. parapertussis, and B. bronchiseptica were studied; they had been grown on nutrient media with prescription combinations. We used casein-coal agar, Borde-Gengou Agar, Regan-Lowe Charcoal Agar as the basis of the nutrient medium. Defibrinated horse, sheep, human donor blood and human donor RBCs were used as a blood component.

Objective. To study antimicrobial effect of extracellular metabolites of probiotic L. plantarum 8P-A3 strain on antibiotic-resistant Klebsiella pneumoniae and Acinetobacter baumannii isolated from infected wounds. Materials and Methods. Bacteriological methods: semiquantitative sector method for extracting opportunistic microorganisms from an infected wound; a method for the identification of the antimicrobial activity of metabolites in a liquid milieu; The identification of bacteria was implemented with the help of the MALDI-TOF Autoflex mass spectrometer (Bruker Daltonics, Germany).

Objective. To analyze and compare multidrug-resistant Klebsiella pneumoniae (KP) strains isolated from different sites of the same patients. Materials and Methods. A total of 30 KP strains isolated from 15 patients treated in intensive care and surgical units in 2020–2021 were studied. Two strains isolated from one child were selected for analyses. One strain was isolated from a clinically significant site (blood/urine/wound/endotracheal aspirate or sputum), the other from a monitoring site (stool).

Objective. To evaluate the ability of bovhyaluronidaze azoximer to destroy biofilms formed by clinical strains of microorganisms. Materials and Methods. The study included 50 clinical strains of Staphylococcus aureus (n = 25), Staphylococcus epidermidis (n = 6), Enterococcus faecalis (n = 8), Escherichia coli (n = 9), Candida albicans (n = 2). Antimicrobial susceptibility testing was performed using broth microdilution method. Biofilm formation culturing in presence of antibiotics, or bovhyaluronidaze azoximer, or their combinations was assessed in Mueller-Hinton broth and brain heart broth in 96-well plates.

Objective. To study the population and genetic structure of S. pneumoniae isolated from children with cystic fibrosis (CF) and congenital malformations of the bronchi and lungs (CM) over the period 2011 to 2021, their serotype diversity in pre-vaccination and post-vaccination periods and susceptibility to antimicrobials. Materials and Methods. A total of 140 S. pneumoniae isolates (during 2011-2021) from respiratory samples of patients with CM or CF were included in the study.

Objective. To develop a technology for producing Russia-made Mueller-Hinton broth and to compare its quality in terms of physicochemical and microbiological parameters with a foreign analog in trials using reference microbial strains sharing common and complex nutritional requirements. Materials and Methods. The work investigated casein acid hydrolysates and Mueller-Hinton broths (MHBs) produced by the Federal Budgetary Institution of Science SRCAMB (MHB–Obolensk) and by BD BBL (MHB–BD) through testing 7 test strains of E.