Personal Experience

Assessment of healthcare workers’ smartphones for microbial contamination

Objective. To assess bacterial contamination of smartphone surfaces belonging to medical staff and medical students who provide round-the-clock medical care in Krasnoyarsk hospitals and who do not work in those institutions. Materials and Methods. Bacterial contamination of 122 smartphones owned by medical staff and medical students was studied in the following groups: doctors (n = 31), nurses (n = 29), students, who work in MIs (n = 27), and students who do not work in MIs (n = 35).

Effect of topical use of solution containing ferrous ions on treatment efficacy in women with recurrent urogenital trichomoniasis

Objective. To assess effect of topical use of solution containing ferrous ions on efficacy of metronidazole therapy in women with recurrent urogenital trichomoniasis. Materials and Methods. The study enrolled 55 women aged of 20–30 years with recurrent trichomoniasis after failures of multiple standard of care regimens. All patients received oral (tablets) and intravaginal (1% gel) metronidazole therapy. Participants in the experimental group (29 women) received intravaginal therapy with a solution of ferrous ions at concentration which was determined as effective for potentiating metronidazole in a previous study.

Development of technology for longterm storage of Mycobacterium tuberculosis cultures

Objective. To develop a technology for freezing and long-term storage of mycobacteria cultures and implement into a microbiological laboratory routine practice. Materials and Methods. For comparison between different condition, the media for freezing mycobacteria containing 0.9% NaCl or Middlebrook’s 7H9 medium with the addition of glycerol at various concentrations: 0%, 4.0%, 20.0%, 50.0% (by final volume) was evaluated. To freeze suspensions of cultures, two methods were used: one – using a cryo-freezer (cooling rate of 1°C/min), the second – direct placement in a deep freeze at a temperature of -80°C.

Functional and antigenspecific serum antibodies in mice after immunization with a candidate vaccine against Shigella flexneri 1b, 2a, 3a, 6, Y

Objective. To determine functional anti-LPS specific serum bactericidal antibody (SBA) and opsonophagocytic killing antibody (OPKA) activities in mice immunized with a pentavalent candidate vaccine against Shigella flexneri 1b, 2a, 3a, 6, Y (PF). Materials and Methods. (CBA x C57 B1/6) F1 mice were immunized with a PF. 14 days after the reimmunization, serum samples were collected and the level of specific IgG in them was determined by ELISA. The sera of mice immunized with individual modified lipopolysaccharides (mLPS), which are part of PF, were used for positive control.

Assessment of biofilmforming activity of Fusarium solani isolated from patients’ skin

Objective. To study pathogenic factors (adhesion and biofilm formation) in F. solani isolated from skin of patients Materials and methods. A total of 86 strains of F. solani isolated from the skin of patients with a diagnosis of L30.3 “Infectious dermatitis” were selected. The reference strain F. solani F-819 was used for quality control. Samples were collected using a sterile cotton swab from an area of 5 × 5 cm.

Safety profile assessment of drug products used for the pathogenetic treatment of COVID19

Objective. To review and summarize literature data in studies of safety of the drug products used for the pathogenetic treatment of COVID-19. Materials and Methods. As the first stage of monitoring the drug’s safety, which are used in the treatment of COVID-19 in Russia, a systematic review of studies of the drug’s safety profiles was carried out: mefloquine, hydroxychloroquine, azithromycin, lopinavir/ritonavir, favipiravir, tocilizumab, olokizumab, baricitinib in the international databases Medline, PubMed, ClinicalTrials.

Comparison of culture and isolation methods for Clostridioides difficile and other anaerobes from stool samples in a routine microbiological laboratory practice

Objective. To improve stool sample culture and isolation of anaerobic flora, including Clostridioides difficile in the routine microbiological laboratory practice at the children’s oncology hospital. Materials and Methods. A total of 517 stool samples collected from patients in children’s oncology hospital from 2013 to 2015 were studied. All samples were analyzed by ELISA for C. difficile toxins and by culture according to dedicated 5 schemes for isolation of anaerobic bacteria, including C.

Cefazolin inoculum effect among methicillinsusceptible Staphylococcus aureus isolated from patients with skin infections

Objective. To evaluate frequency and intensity of cefazolin inoculum effect among methicillin-susceptible staphylococci isolated from patients with skin infections. Materials and Methods. A total of 80 methicillin susceptible isolates of Staphylococcus aureus were identified by cefoxitin disk-diffusion test and negative results of real-time PCR for mecA gene. Inoculum effect was measured by broth microdilution test with two inocula with concentrations of 5 × 105 CFU/mL and 5 × 107 CFU/mL.

Local microbiological monitoring as a basis for determining etiological significance of conditional pathogens: data from a burn intensive care unit

Objective. To assess the etiology of infections, microbial associations and antimicrobial resistance in a burn intensive care unit. Materials and Methods. A microbiological study of 1322 biological samples from 195 patients with extensive burns included 479 blood samples, 82 respiratory samples, 326 urine samples, and 435 wound samples. Antimicrobial susceptibility testing was performed, and coefficients of constancy and associativity (CA), as well as the Jaccard coefficient were calculated. Results. The etiology of infections was represented by: Pseudomonas aeruginosa – 23%, Acinetobacter baumannii – 19.

In vitro activity of thiamphenicol against Haemophilus influenzae, Streptococcus pneumoniae and Streptococcus pyogenes clinical isolates

Objective. To determine in vitro activity of thiamphenicol and other clinically available antimicrobials against clinical isolates of Haemophilus influenzae, Streptococcus pneumoniae and Streptococcus pyogenes. Materials and Methods. We included in the study 875 clinical isolates from 20 Russian cities during 2018–2019. Among tested strains, 126 were H. influenzae, 389 – S. pneumoniae, 360 – S. pyogenes. Antimicrobial susceptibility testing was performed using broth microdilution method according to ISO 20776-1:2006. AST results were interpreted according to EUCAST v.