Antimicrobial Susceptibility of Group A Streptococci of Different emm Genotypes, Isolated from Patients with Invasive and Non-Invasive Skin and Soft Tissue Infections

Clinical Microbiology and Antimicrobial Chemotherapy. 2015; 17(1):67-72

​ Briko N.I., Dmitrieva N.F., Kleymenov D.A., Lipatov K.V., Glushkova E.V., Kotin V.V.
Streptococcus pyogenes antimicrobial resistance genotype
Section
Personal Experience
Type
Journal article
Publication
Clinical Microbiology and Antimicrobial Chemotherapy. 2015; 17(1):67-72

Objective.

Aim. To evaluate the antimicrobial susceptibility of group A streptococci (GAS) of different emm-genotypes, isolated from patients with invasive and non-invasive skin and soft tissue infections.

Materials and Methods.

Overall 91 strains of GAS isolated during 2008–2011 from patients with invasive and non-invasive skin and soft tissue infections hospitalized in one Moscow’s hospital were included in the study. Identification was done by latex agglutination, genotyping — by CDC protocol. Susceptibility to antimicrobials was evaluated by broth microdilution method in accordance with EUCAST v.4.1 recommendations.

Results.

Resistance to tetracycline was noted in 50,5% of strains; to azithromycin, clarithromycin and erythromycin — in 17.6%, 15.4% and 15.4% of strains, respectively; 13,2%, 5,5% and 1,1% of strains were non-susceptible to chloramphenicol, clindamycin and levofloxacin. Among tested strains 23.1% were resistant to more than one antimicrobial. Out of 35 different emm genotypes multiresistance was detected in the following emm genotypes: 44.0, 49.8, 65.0, 74.0, 88.2, st 1731.2.

Conclusions.

The most prominent antimicrobial resistance problem in the tested strains was very high rate on non-susceptibility to tetracycline. All isolates tested, independently of type of infection (invasive and non-invasive) were highly susceptible to penicillin G. Multiresistant Gas isolates were belonging to few international emmгgenotypes.

Nikolay I. Briko

I.M. Sechenov First Moscow State Medical University, Moscow, Russia

Dmitrieva N.F.

I.M. Sechenov First Moscow State Medical University, Moscow, Russia

Kleymenov D.A.

I.M. Sechenov First Moscow State Medical University, Moscow, Russia

Lipatov K.V.

I.M. Sechenov First Moscow State Medical University, Moscow, Russia

Glushkova E.V.

I.M. Sechenov First Moscow State Medical University, Moscow, Russia

Kotin V.V.

I.M. Sechenov First Moscow State Medical University, Moscow, Russia

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