Clinical Microbiology and Antimicrobial Chemotherapy. 2025; 27(3):417-424
Objective. To develop and conduct the first stage of validation for a PCR test that identifies key mutations in the V domain of the 23S rRNA gene of M. pneumoniae (A2058G, A2059G, and A2062C). This test allows for the identification of macrolide-resistant M. pneumoniae strains in samples of native biological material.
Materials and Methods. The study included a panel of M. pneumoniae DNA samples (n = 107) from the collection of the DeMaRes multicenter study, as well as native material (n = 52).