multilocus sequence typing

The genetic characteristics of Neisseria meningitidis causing invasive meningococcal infections in Smolensk region

Objective. To determine antigenic and genetic characteristics of Neisseria meningitidis isolated from the patients with invasive meningococcal infections in Smolensk region in comparison with those circulating in other regions. Materials and Methods. A total of 14 cerebrospinal fluid samples obtained in 2016–2019 and containing N. meningitidis DNA were tested using AmpliSens® Nm-ABCW kit, multilocus sequence typing and antigenic finetyping of outer membrane proteins. The results were analyzed by the BURST algorithm and other functionalities available at PubMLST.

Methodology Issues of Legionella Pneumonia Diagnosis in Medical Institutions (Part 2)

Objective. To use algorithm of microbiologic and epidemiologic methods when detecting two or more cases of legionellosis in the medical institution, in accordance with the international guidelines and to assess its efficacy. Materials and Methods. A total of 76 hematological patients with pneumonia and acute respiratory failure were tested for legionellosis using bronchoalveolar lavage (BAL) fluid culture and Legionella urinary antigen test between December 2010 and January 2013. Water samples obtained from the institution’s hot water supply system were tested for Legionella by the standard microbiology method.

Phenotypic and Genotypic Characteristics of Pneumococci Isolated from Patients with Pneumococcal Meningitis

Antimicrobial susceptibility, serotype distribution, and multilocus sequence types of Streptococcus pneumoniae isolated from the patients with meningitis were determined. We tested a total of 84 isolates which have been obtained in Moscow during …