Objective. To assess the possibility of standard disk application as an antibiotic source for determination of the minimal inhibitory concentrations (MICs) by serial microdilution method.
Materials and Methods. A simple method of working solutions preparation using standard paper discs as an antibiotics source available in most microbiological laboratories is proposed. A multiple determination of antibiotic MICs for E. coli ATCC 25922 and P. aeruginosa ATCC 27853 strains was conducted.