microbiome

Objective. To assess quality of bacterial detection using 16S rRNA gene sequencing with accredited control materials designed for external quality control of PCR studies. Materials and Methods. Control materials obtained as part of the ISI «FSVOK» program: «DNA detection of Mycoplasma hominis, Ureaplasma spp., Ureaplasma urealyticum, Ureaplasma parvum by PCR (STI)», were analyzed by 16S rRNA gene sequencing. The control sets of samples were presented with lyophilized solutions of M.

Objective. To assess practicing dentists who treat patients conservatively attitude to antibiotic therapy in general, the degree of awareness of the rules for prescribing antibiotics, approaches to prescribing antibiotics for various dental diseases and therapeutic and preventive manipulations. Materials and Methods. The questionnaire was developed and an anonymous survey-questionnaire of 173 practical dentists conducting therapeutic reception of patients was carried out. The questionnaire included a list of diseases, therapeutic and prophylactic manipulations in which respondents consider the use of antibiotics indicated and questions to assess the respondents’ awareness of modern methods of antibiotic therapy and their views on the rules for prescribing antibiotics.