Objective. To study the genetic diversity of vancomycin-resistant Enterococcus faecium (VR-E. faecium) isolated from the blood culture in patients with hematological malignancies by multilocus sequence typing (MLST).
Materials and Methods. VR-E. faecium isolated from the blood culture in hematological patients in 6 hospitals of 4 Russian cities (2003–2019) were evaluated. Susceptibility to vancomycin was tested by the broth microdilution method (CLSI, 2018). Vancomycin-resistance genes (vanA, vanB) were identified by polymerase chain reaction.
Posaconazole is a broad-spectrum triazole antifungal agent with potent activity against various fungi, including yeast and molds, including Mucorales, what makes a key difference with itraconazole and voriconazole. Clinical studies have demonstrated …
Objective. To evaluate the prevalence of acquired carbapenemase genes among A. baumannii isolates collected from blood culture in patients with hematological malignancies.
Materials and Methods. This prospective multicenter study included A. baumannii isolated from blood culture in hematological patients in 7 Russian hospitals (2003–2015). Minimum inhibitory concentrations (MICs) of meropenem and imipenem were determined by broth microdilution method (CLSI, 2017). All A. baumannii isolates were tested by multiplex real-time PCR for presence of acquired carbapenemase genes (blaOXA-23-like, blaOXA-24⁄40-like, blaOXA-58-like, blaNDM-like, blaVIM-like and blaIMP-like).
Objective. The objective of the study was to evaluate the prevalance of genes blaTEM, blaSHV and blaCTX-M among extended-spectrum beta-lactamase (ESBL)-producing Enterobacterales isolates collected from blood culture in patients with hematological malignancies.
Materials and methods. The prospective multicenter study included Enterobacterales isolates collected from blood culture in hematological patients from 10 Russian hospitals (2003-2015). The isolates were screened for ESBLs by the phenotypic methods (CLSI 2017). Genes encoding TEM, SHV and CTX-M were detected using polymerase chain reaction (PCR) by specific primers.
Objective. To evaluate antimicrobial susceptibility of Enterococcus spp. isolated from blood culture in patients with hematological malignancies.
Materials and Methods. Antimicrobial susceptibility of 427 Enterococcus spp. collected from 10 hospitals in 8 cities of Russia in 2002-2016 as part of the multicenter study was tested by the broth microdilution method [CLSI 2015].
Results. Among bloodstream pathogens there was a prevalence of E. faecium (78.2%), followed by E. faecalis (19.7%) and other Enterococcus spp.
Objective. To evaluate the biofilm formation by isolates of Candida spp. obtained from blood culture in patients with or without hematological malignancies.
Materials and Methods. Biofilm formation by Candida spp. was determined by the method of Pierce C. and Tumbarello M. Isolates with optical density (OD) >0.1 were considered as biofilm-forming.
Results. A total of 109 isolates of Candida spp. (C. albicans [n=22], C. parapsilosis [n=22], C. tropicalis [n=22], C.