flucytosine

Objective. To develop an in vitro model for the development of Candidozyma auris resistance to flucytosine (5-FC) and characterize its associated mutational profile. Materials and Methods. An adaptive laboratory evolution study was performed on seven clinical C. auris isolates of the South Asian clade obtained from independent epidemiological foci in Russian hospitals. Isolates underwent stepwise selection with increasing 5-FC concentrations, with molecular verification at defined checkpoints. Minimum inhibitory concentrations (MICs) were determined by broth microdilution (EUCAST).