Potentiation of antimicrobial activity of colistin with antibiotics of different groups against multidrug- and extensively drug-resistant strains of Klebsiella pneumoniae, Acinetobacter baumannii and Pseudomonas aeruginosa

Objective. To reveal antibiotics being capable of potentiating the antimicrobial activity of colistin against multidrug- and extensively drug-resistant strains of Klebsiella pneumoniae, Acinetobacter baumannii and Pseudomonas aeruginosa. Materials and Methods. The minimum inhibitory concentrations (MIC) of colistin alone and in combination with fixed concentrations of antibiotics of different groups were determined for 272 multidrug- and extensively drug-resistant strains of K. pneumoniae, A. baumannii and P. aeruginosa. Bactericidal activity of colistin, carbapenems, clarithromycin and their combinations were also determined at fixed PK/PD breakpoint concentrations of antibiotics.

Detection of acquired carbapenemase genes among Acinetobacter baumannii isolated from blood culture in patients with hematological malignancies

Objective. To evaluate the prevalence of acquired carbapenemase genes among A. baumannii isolates collected from blood culture in patients with hematological malignancies. Materials and Methods. This prospective multicenter study included A. baumannii isolated from blood culture in hematological patients in 7 Russian hospitals (2003–2015). Minimum inhibitory concentrations (MICs) of meropenem and imipenem were determined by broth microdilution method (CLSI, 2017). All A. baumannii isolates were tested by multiplex real-time PCR for presence of acquired carbapenemase genes (blaOXA-23-like, blaOXA-24⁄40-like, blaOXA-58-like, blaNDM-like, blaVIM-like and blaIMP-like).

Genotypes and metallo-beta-lactamases carriage in carbapenem-resistant Pseudomonas aeruginosa isolated from children in Moscow

Objective. To characterize the population structure and determine the genetic mechanisms of carbapenem resistance in nosocomial isolates of P. aeruginosa collected in Moscow in 2012-2016. Materials and methods. Carbapenem-resistant isolates were collected in two pediatric hospitals in Moscow. Antibiotics susceptibility of P. aeruginosa isolates was assessed using the E-tests and disk-diffusion method. Multilocus sequence typing (MLST) was used for genotyping of the isolates. The presence of metallo-betalactamase (MBL) genes was determined using real-time PCR.

Bacteriophage Preparations and Antibiotic Combinations: In vitro Activity Against Extensively Drug-Resistant Isolates of Pseudomonas aeruginosa ST235

The activity of 11 antibiotics combinations and 4 commercially available preparations for phagotherapy in regard to metallo-beta-lactamase (MBL) producing Pseudomonas aeruginosa clinical isolates of sequence type 235 (ST 235) belonging to the clonal …

Production of Carbapenemases in Klebsiella pneumoniae Isolated in Saint-Petersburg

Carbapenem-resistant nosocomial K. pneumoniae isolates are a significant problem in clinical practice. Carbapenem resistance in K. pneumoniae isolated from hospitalized patients was 2.8% over the study period. NDM-1 carbapenemase-producing strains …

Genotyping Analysis of Nosocomial Acinetobacter baumannii Strains

The problem of genotypic variety of Acinetobacter baumannii isolated from patients with healthcare associated infections (HCAIs) are discussed. Carbapenemresistant strains, which are predominant in the study region, were shown to belong to the …

«Checkerboard Array» as a Test for Evaluation of Decrease in Gram-Negative Bacteria Resistance to Carbapenems in the Presence of Bisphosphonate

One of the main mechanisms of resistance of Gramnegative microorganisms to antibiotics is enzymatic hydrolysis by beta-lactamases (for example, metallobeta-lactamases). Searching of paths of strengthening of carbapenems action is possible due to …

Carbapenems in the Multidisciplinary Hospital: Some Clinical and Economic Consequences

The changes in carbapenem consumption during 6 consecutive years have been studied in retrospective trial. 88% of carbapenems have been administered in the ICU’s and hematology department. The 3-fold increase of the total consumption during …

Wound Infections: a Role of Microbiological Monitoring for the Hospital Antimicrobial Policy

During the last 7 years the leading pathogens in the etiology of skin and soft tissue infections in the hospital were Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli and Pseudomonas аeruginosа. Statistical prognosis does not suggest …

Hospital-Acquired Pneumonia in Patients with Hematological Malignancies in an ICU

Objective. To determine causative agents of hospitalacquired pneumonia (HAP), their incidence rates, and susceptibility to antimicrobial agents in patients with hematological malignancies in an intensive care unit (ICU). Materials and Methods. Broncho-alveolar lavage (BAL) specimens obtained from oncohaematologic patients with pneumonia hospitalized to the ICU between December 2009 and December 2010 were retrospectively studied. Results. A total of 142 BAL specimens were obtained from 72 hospitalized patients aged of 19 to 86 years.