bacteriocins

Objective. Conducting a whole-genome study and bioinformatic analysis of a Lacticaseibacillus strain to confirm taxonomic affiliation, identify genetic determinants of metabolic activity, stress resistance, and bacteriocin production as a criterion for the standardization of mono- and multi-component probiotics. Materials and Methods. The object of the study was a strain isolated from a coloproctology patient in 2024. Cultivation was carried out on MRS medium under microaerophilic conditions. Wholegenome sequencing was performed on the Oxford Nanopore Technologies MinION platform (R10.

Objective. To study antimicrobial effect of extracellular metabolites of probiotic L. plantarum 8P-A3 strain on antibiotic-resistant Klebsiella pneumoniae and Acinetobacter baumannii isolated from infected wounds. Materials and Methods. Bacteriological methods: semiquantitative sector method for extracting opportunistic microorganisms from an infected wound; a method for the identification of the antimicrobial activity of metabolites in a liquid milieu; The identification of bacteria was implemented with the help of the MALDI-TOF Autoflex mass spectrometer (Bruker Daltonics, Germany).