23S rRNA

Objective. To develop and conduct the first stage of validation for a PCR test that identifies key mutations in the V domain of the 23S rRNA gene of M. pneumoniae (A2058G, A2059G, and A2062C). This test allows for the identification of macrolide-resistant M. pneumoniae strains in samples of native biological material. Materials and Methods. The study included a panel of M. pneumoniae DNA samples (n = 107) from the collection of the DeMaRes multicenter study, as well as native material (n = 52).

Objective. To identify point mutations in 23S rRNA gene associated with phenotypic drug resistance to clarithromycin (CLR) in clinical isolates of H. pylori. Materials and Methods. A total of 50 H. pylori clinical isolates (2014–2022) from adult patients with chronic gastritis (n = 32), duodenal ulcer (n = 11) and gastric cancer (n = 1) were included in this study. Of 50 isolates, 30 were obtained from newly diagnosed patients, 20 – from previously treated patients after eradication failure.

Objective. To determine spectrum and prevalence of genetic determinants of resistance to macrolides in Mycoplasma genitalium in a Russian patient population. Materials and Methods. A total of 873 M. genitalium-positive samples from five geographical regions of Russia were investigated over the period of 2009–2019 using the previously developed protocol of real-time polymerase chain reaction (allows detecting any nucleotide substitutions in the 23S rRNA gene of M. genitalium at positions 2058, 2059, and 2611).

Mycoplasma pneumoniae is a bacterial pathogen cause of upper and lower respiratory tract infections, transmitted by airborne droplets, causing outbreaks of pneumonia mainly in closed groups. According with recommendations, M. pneumoniae infections …