Personal Experience

Objective. To present the data on the main mechanism of molecular variation in P. aeruginosa causing chronic lung infection in patients with cystic fibrosis. Materials and Methods. A total of 1800 throat swabs and sputum samples from cystic fibrosis patients were included in the study over the 10-year period. P. aeruginosa isolates were primarily identified by the biochemical method using the API 20NE test strips (bioMerieux, France). Antimicrobial susceptibility testing was performed by disc diffusion method.

This paper presents a clinical case of large focal liver mass in a 55-year old patient with HCV-associated liver cirrhosis and prolonged high-dose alcohol exposure. Given the inconsistency between clinical data and ultrasound examination results as …

Objective. To estimate composite indicators of systemic antibiotic consumption in the hospital segment of Saint-Petersburg in 2014–2018. Materials and Methods. Information on the volume and structure of AMP consumption in the hospital segment of St. Petersburg in 2014–2018 was obtained from AlfaRm Databases. The amount of purchased AMP was recalculated in the number of average daily maintenance doses (Defined Daily Dose, DDDs), as well as DDDs per 100 bed-days (DDDh) for each international nonproprietary name of AMP.

Objective. To study antimicrobial susceptibility of Streptococcus pneumoniae clinical isolates obtained from the patients with community-acquired pneumonia in the Central Kazakhstan. Materials and Methods. A total of 186 clinical strains of S. pneumoniae were obtained from the patients with respiratory tract infections in the Central Kazakhstan over the period of 2012–2017. Antimicrobial susceptibility was determined by disk-diffusion method. The isolation and identification of pathogens was performed by routine methods. Antimicrobial (oxacillin, vancomycin, clindamycin, azithromycin, chloramphenicol, tetracycline, rifampin, levofloxacin, linezolid, trimethoprim/sulfamethoxazole) susceptibility testing was performed and interpreted according to CLSI criteria (M100-24).

The options for the use of «Plot By» tab in the web platform AMRmap are reviewed in the article. Description of some functionality is supported by the specific examples, and Klebsiella pneumoniae was selected as a study object. The analysis elements …

Objective. To study the colonization activity of clinical strains of uropathogenic Escherichia coli (UPEC) against various types of urological catheters. Materials and Methods. Biofilms of E. coli (n = 6) were formed for 24 h according to the standard method. Urological catheters made from latex, polyvinylchloride, silicon and silver-coated silicon were used as a surface for bacterial colonization. The surface roughness and hydrophobicity, biofilm mass, number of colony forming units in biofilm, and frequency of conjugation were assessed.

Objective. To study genetic diversity of Klebsiella pneumoniae strains obtained in the National Medical and Surgical Center named after N.I. Pirogov (Moscow) using next-generation sequencing. Materials and Methods. A total of 19 isolates of K. pneumoniae were included in the study (18 – from patients and 1 – from nosocomial environment). The strains were isolated from January 30 to October 9, 2017 and phenotypically showed multi-drug resistance. DNA sequencing of the strains was performed on the Illumina HiSeq1500 instrument using the Illumina HiSeq PE Rapid Cluster Kit v2 and Illumina HiSeq Rapid SBS Kit v2 kits.

Objective. To evaluate a level of physicians’ awareness of the specific issues of epidemiology, outcomes, prevention and treatment of chronic hepatitis B and C. Materials and Methods. The anonymous questioning was performed among 120 physicians (record of service in a given specialty ranged from 8.1 to 18.6 years) in Kirov city and region. The participants were represented by the following specialties: pediatricians – 41.7% (n=50), obstetricians/gynecologists – 35.8% (n=43), and dentists – 22.

Objective. To study the etiological role and antimicrobial susceptibility of Pseudomonas aeruginosa in one tertiary care hospital with analysis of the influence of antimicrobial stewardship implementation. Materials and Methods. In 2013 primary microbiological evaluation was performed. In 2014 antimicrobial stewardship program was implemented. The impact of the introduction of microbiological monitoring system and antimicrobial stewardship program was evaluated. Results. In total, 461 P. aeruginosa strains were isolated during the study period.

Objective. To evaluate the results of accelerated identification of microorganisms isolated from positive hemocultures obtained from patients of various departments of a large multi-profile hospital by MALDI-TOF MS and to compare them with classical cultural methods. Materials and Methods. In the period from 2017 to 2018, the study included 109 positive blood cultures from 73 patients from UIA GKB number 40 at the age of 1 to 83 years. Blood samples were taken in aseptic conditions from 2 peripheral veins according to the standard protocol in 12 departments of the health facility.