Personal Experience

Listeriosis has a variety of clinical manifestations from febrile gastroenteritis to bacteremia, sepsis, meningoencephalitis and infective endocarditis with valvular heart disease. In this report, we present a rare case of successful treatment of …

Objective. To determine the effect of the tinctorial properties of microorganism on the nutritional status and the early outcome in patients with systemic inflammatory response syndrome (SIRS) of bacterial origin. Materials and Methods. A retrospective statistical analysis of case histories of 137 patients with signs and symptoms of SIRS and bacterial infections (caused by a single pathogen) was performed. The following clinical and laboratory parameters of protein-energy undernutrition (PEU) were determined and assessed: body mass index, body weight deficit, total protein, serum albumin, absolute peripheral blood lymphocyte count.

Objective. To identify mutations in UL97 gene associated with antiviral drug resistance in recipients of allogeneic hematopoietic stem cells transplants (allo-HSCT). Materials and Methods. A total of 9 HCMV DNA samples were studied. These samples were received from the blood of 8 allo-HSCT recipients who were infected with HCMV and undergoing treatment at NMRC of Hematology (Russia) over the period of 2016 to 2017. Sanger sequencing was used to find mutations.

Objective. To present the data on the main mechanism of molecular variation in P. aeruginosa causing chronic lung infection in patients with cystic fibrosis. Materials and Methods. A total of 1800 throat swabs and sputum samples from cystic fibrosis patients were included in the study over the 10-year period. P. aeruginosa isolates were primarily identified by the biochemical method using the API 20NE test strips (bioMerieux, France). Antimicrobial susceptibility testing was performed by disc diffusion method.

This paper presents a clinical case of large focal liver mass in a 55-year old patient with HCV-associated liver cirrhosis and prolonged high-dose alcohol exposure. Given the inconsistency between clinical data and ultrasound examination results as …

Objective. To estimate composite indicators of systemic antibiotic consumption in the hospital segment of Saint-Petersburg in 2014–2018. Materials and Methods. Information on the volume and structure of AMP consumption in the hospital segment of St. Petersburg in 2014–2018 was obtained from AlfaRm Databases. The amount of purchased AMP was recalculated in the number of average daily maintenance doses (Defined Daily Dose, DDDs), as well as DDDs per 100 bed-days (DDDh) for each international nonproprietary name of AMP.

Objective. To study antimicrobial susceptibility of Streptococcus pneumoniae clinical isolates obtained from the patients with community-acquired pneumonia in the Central Kazakhstan. Materials and Methods. A total of 186 clinical strains of S. pneumoniae were obtained from the patients with respiratory tract infections in the Central Kazakhstan over the period of 2012–2017. Antimicrobial susceptibility was determined by disk-diffusion method. The isolation and identification of pathogens was performed by routine methods. Antimicrobial (oxacillin, vancomycin, clindamycin, azithromycin, chloramphenicol, tetracycline, rifampin, levofloxacin, linezolid, trimethoprim/sulfamethoxazole) susceptibility testing was performed and interpreted according to CLSI criteria (M100-24).

The options for the use of «Plot By» tab in the web platform AMRmap are reviewed in the article. Description of some functionality is supported by the specific examples, and Klebsiella pneumoniae was selected as a study object. The analysis elements …

Objective. To study the colonization activity of clinical strains of uropathogenic Escherichia coli (UPEC) against various types of urological catheters. Materials and Methods. Biofilms of E. coli (n = 6) were formed for 24 h according to the standard method. Urological catheters made from latex, polyvinylchloride, silicon and silver-coated silicon were used as a surface for bacterial colonization. The surface roughness and hydrophobicity, biofilm mass, number of colony forming units in biofilm, and frequency of conjugation were assessed.

Objective. To study genetic diversity of Klebsiella pneumoniae strains obtained in the National Medical and Surgical Center named after N.I. Pirogov (Moscow) using next-generation sequencing. Materials and Methods. A total of 19 isolates of K. pneumoniae were included in the study (18 – from patients and 1 – from nosocomial environment). The strains were isolated from January 30 to October 9, 2017 and phenotypically showed multi-drug resistance. DNA sequencing of the strains was performed on the Illumina HiSeq1500 instrument using the Illumina HiSeq PE Rapid Cluster Kit v2 and Illumina HiSeq Rapid SBS Kit v2 kits.