Clinical Microbiology and Antimicrobial Chemotherapy. 2016; 18(3):212-225
Objective. To characterize gut microbiota composition in patients with different body weight.
Materials and Methods. A total of 92 patients (mean age: 52.8 years) with different body mass index (BMI), with or without abdominal obesity were enrolled in the study. A metagenomic analysis as an assessment of 16S rRNA gene diversity in a total DNA sample was performed according to 16S Metagenomic Sequencing Library Preparation protocol. Taxonomic composition of the samples was evaluated using 16S rRNA gene sequences database Greengenes v.