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Lactobacilli Species Diversity in Different States of Vaginal Microbiota in Pregnant Women

Objective. To determine lactobacilli species diversity in different states of vaginal microbiota in pregnant women using culture, mass-spectrometry, and DNAbased assays. Materials and Methods. A total of 163 pregnant women (18 to 40 years of age) were examined in the 2nd or 3rd trimester. A comprehensive microbiological examination of vaginal swabs, including Gram staining, methylene blue staining, and culture was performed. All isolated microorganisms were identified to species level using mass-spectrometry.