Impact of nutrient medium composition on protein profiling of Bordetella spp.

Objective.

To perform comparative analysis of Bordetella protein profiling data and their dependence on various nutrient media.

Materials and Methods.

This prospective open single-center study, the protein profiles of B. pertussis, B. parapertussis, and B. bronchiseptica were studied; they had been grown on nutrient media with prescription combinations. We used casein-coal agar, Borde-Gengou Agar, Regan-Lowe Charcoal Agar as the basis of the nutrient medium. Defibrinated horse, sheep, human donor blood and human donor RBCs were used as a blood component. Penicillin, benzathine benzylpenicillin, cephalexin and antibiotic-free formulation were used as antimicrobial component. A total of 48 prescription combinations were tested. Identification of proteomic profiles of Bordetella spp. was carried out on MALDI-TOF mass spectrometer using Standard mode according to “Score” indicator.

Results.

The quantitative values of the “Score” indicator were subjected to comparative analysis depending on the used base, blood, antimicrobial, and combinations of individual components. According to “Score” indicator, the most reliable identification of Bordetella spp. was achieved using Borde-Gengou Agar basis (p = 0.035, Kruskal – Wallis test). No statistically significant differences were found when using other components.

Conclusions.

The use of Borde-Gengou Agar base is the most appropriate for mass spectrometric identification of Bordetella spp.