Genomic properties in Achromobacter spp. strains from cystic fibrosis patients in Russia

Clinical Microbiology and Antimicrobial Chemotherapy. 2021; 23(3):220-225

​ Bocharova Y.A., Saviniova T.A., Chaplin A.V., Lyamin A.V., Kondratenko O.V., Polikarpova S.V., Zhilina S.V., Fedorova N.I., Korzhanova M., Mayansky N.A., Chebotar I.V.
10.36488/cmac.2021.3.220-225
Achromobacter cystic fibrosis antimicrobial resistance genes sequence type
Section
Diseases and Pathogens
Type
Original Article
Publication
Clinical Microbiology and Antimicrobial Chemotherapy. 2021; 23(3):220-225

Objective.

To determine species, sequence-types, antimicrobial resistance and virulence genes in Achromobacter spp. isolates obtained from cystic fibrosis (CF) patients in Russia.

Materials and Methods.

Samples (sputum, nasopharyngeal swab) from 168 CF patients from 48 regions were studied. Whole-genome sequencing (WGS) was performed on MGISEQ-2000 platform. SPAdes software, Galaxy, ResFinder, Integrall, PubMLST were used for analysis of WGS data.

Results.

A total of 18 strains of Achromobacter spp. were isolated from 16 of 168 CF patients.Achromobacter xylosoxidans was the most prevalent and detected in 13⁄18 cases (72%). Studied Achromobacter spp. isolates belonged to 14 sequence types, including 8 new sequence types. An adaptive resistance gene carriage was a rare phenomenon (1⁄18 isolates).

Conclusions.

The Achromobacter spp. colonization rate of respiratory system in CF patients in Russia corresponds to the data reported in other countries. A. xylosoxidans isolates were the most prevalent (72%). Achromobacter spp. isolates from CF patients in Russia and show a high clonal diversity.

Bocharova Y.A.

Pirogov Russian National Research Medical University, Moscow, Russia

Saviniova T.A.

Pirogov Russian National Research Medical University, Moscow, Russia

Chaplin A.V.

Pirogov Russian National Research Medical University, Moscow, Russia

Artem V. Lyamin

Samara State Medical University, Samara, Russia

Kondratenko O.V.

Samara State Medical University, Samara, Russia

Polikarpova S.V.

Moscow City Clinical Hospital named after O.M. Filatov, Moscow, Russia

Zhilina S.V.

Morozov Moscow City Pediatric Hospital, Moscow, Russia

Fedorova N.I.

Pirogov Russian National Research Medical University, Moscow, Russia

Korzhanova M.

Pirogov Russian National Research Medical University, Moscow, Russia

Nikolay A. Mayansky

Pirogov Russian National Research Medical University, Moscow, Russia

Igor V. Chebotar

Pirogov Russian National Research Medical University, Moscow, Russia

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