Investigation of a candidemia outbreak caused by C. parapsilosis in a pediatric hematology/oncology center

Clinical Microbiology and Antimicrobial Chemotherapy. 2020; 22(4):266-270

​ Kozhushnaya O.S., Solopova G.G., Voropaev A.D., Markova Zh.V., Satsuk A.V., Balamozhnova A.O., Novichkova G.A.
10.36488/cmac.2020.4.266-270
сandidemia Candida parapsilosis immunocompromised children short tandem repeats microsatellite repeats
Section
Diseases and Pathogens
Type
Original Article
Publication
Clinical Microbiology and Antimicrobial Chemotherapy. 2020; 22(4):266-270

Objective.

To investigate a candidemia outbreak caused by C. parapsilosis in a clinical unit of the Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology (NMRC PHOI).

Materials and Methods.

A total of 35 isolates of C. parapsilosis obtained from clinically significant specimens and swabs, including hands of nursing staff of the NMRS PHOI, over the 2018-2020 were genotyped in this retrospective study. Identification of C. parapsilosis isolates was performed by microbiological methods. The clonal structure of C. parapsilosis isolates was investigated by polymerase chain reaction followed by fragment analysis of microsatellite repeats (short tandem repeats, STR markers).

Results.

The results of the study showed genetic diversity of the population of C. parapsilosis isolates over the 2018–2020 in the NMRC PHOI. A total of 27 genotypes were identified, one of which caused candidemia in 6 patients.

Conclusions.

The study results confirmed the nosocomial candidemia outbreak and showed the fragment analysis of STR-markers may be used for epidemiological investigations of outbreaks in hospital settings.

Kozhushnaya O.S.

Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia

Galina G. Solopova

Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia

Voropaev A.D.

Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia

Markova Zh.V.

Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia

Satsuk A.V.

Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia

Balamozhnova A.O.

Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia

Novichkova G.A.

Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia

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