Antimicrobial resistance of Haemophilus influenzae clinical isolates in Russia: the results of multicenter epidemiological study «PEHASus 2014–2017»

Clinical Microbiology and Antimicrobial Chemotherapy. 2019; 21(4):317-323

​ Ivanchik N.V., Sukhorukova M.V., Chagaryan А.N., Dekhnich А.V., Kozlov R.S., Arkhipenko М.V., Bekker G.G., Ershova М.G., Zholobova А.F., Zubareva N.А., Ishakova L.М., Kirillova G.Sh., Krechikova О.I., Lazareva А.V., Morozova О.А., Moskvitina Е.N., Petrova Т.А., Sivaya О.V., Chernyavskaya Yu.L.
10.36488/cmac.2019.4.317-323
Haemophilus influenzae antimicrobial resistance antimicrobial susceptibility epidemiology
Section
Antimicrobial Resistance
Type
Original Article
Publication
Clinical Microbiology and Antimicrobial Chemotherapy. 2019; 21(4):317-323

Objective.

To evaluate antimicrobial resistance patterns of clinical Haemophilus influenzae isolates from different regions of Russia during 2014–2017.

Materials and Methods.

We included in the study 185 clinical H. influenzae isolates from 13 Russian cities. Susceptibility testing was performed by reference broth microdilution method (ISO 207761:2006). Susceptibility testing results were interpreted using EUCAST v. 10.0 breakpoints.

Results.

H. influenzae isolates were highly susceptible to all (96.8%-100%) β-lactams tested (amoxicillin/ clavulanate, cefixime, ceftaroline, ceftibuten, cefditoren, ertapenem) with the exception of ampicillin (15.1% isolates were resistant). Fluoroquinolones, – ciprofloxacin, levofloxacin, moxifloxacin were active against 95.7%, 94.6% and 97.9% of isolates, respectively. Chloramphenicol was active against 97.9% of isolates, tetracycline – against 98.4% of isolates. Activity of trimethoprim/sulfamethoxazole was low (69.2% of susceptible isolates).

Conclusions.

In general, we can state the favorable situation with the susceptibility of H. influenzae to antimicrobials in Russia. However, the increasing of resistance to aminopenicillins and emerging of quinolone-resistant strains could become a problem in the future. Study Results are deposited at on-line resource AMRmap (http://AMRmap.ru).

Nataly V. Ivanchik

Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, Smolensk, Russia

Marina V. Sukhorukova

Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, Smolensk, Russia

Chagaryan А.N.

Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, Smolensk, Russia

Dekhnich А.V.

Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, Smolensk, Russia

Roman S. Kozlov

Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, Smolensk, Russia

Arkhipenko М.V.

Research Institute – Regional Clinical Hospital No.1 named under S.V. Ochapovskiy, Krasnodar, Russia

Bekker G.G.

Clinical Hospital at the Novosibirsk-Main Station, Novosibirsk, Russia

Ershova М.G.

Infectious Diseases Clinical Hospital, Yaroslavl, Russia

Zholobova А.F.

Tolyatti City Clinical Hospital No.5, Tolyatti, Russia

Zubareva N.А.

Perm State Medical University, Perm, Russia

Ishakova L.М.

Republican Clinical Hospital, Kazan, Russia

Kirillova G.Sh.

Emergency Care Hospital, Naberezhnye Chelny, Russia

Krechikova О.I.

Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, Smolensk, Russia

Lazareva А.V.

National Medical Research Center for Children's Health, Moscow, Russia

Morozova О.А.

I.M. Sechenov First Moscow State Medical University, Moscow, Russia

Moskvitina Е.N.

Siberian Federal Scientific Clinical Center, Seversk, Russia

Petrova Т.А.

City Clinical Hospital No.1, Smolensk, Russia

Sivaya О.V.

Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, Smolensk, Russia

Chernyavskaya Yu.L.

Murmansk Regional Clinical Hospital, Murmansk, Russia

  • 1. World Health Organization (WHO). Antimicrobial Resistance: Global Report on Surveillance 2014; Antimicrobial Resistance Global Surveillance Report. Geneva, Switzerland: WHO; 2014.
  • 2. Giufrè M., Fabiani M., Cardines R., Riccardo F., Caporali M.G., D’Ancona F., et al. Increasing trend in invasive non-typeable Haemophilus influenzae disease and molecular characterization of the isolates, Italy, 2012-2016. Vaccine. 2018;36(45):66156622. DOI: 10.1016/j.vaccine.2018.09.060
  • 3. Handbook of microbiological media / by Roland M. Atlas. – 2nd ed.: CRS Press; 1997. 1946 p.
  • 4. European Committee on Antimicrobial Susceptibility testing (EUCAST). Breakpoint tables for interpretation of MICs and zone diameters. Ver.10.0. Аvailable at: www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/Breakpoint_tables/v10.0.
  • 5. GOST R ISO 20776-1-2010. Clinical laboratory testing and in vitro diagnostic test systems. Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices. Part 1. Reference method for testing the in vitro activity of antimicrobial agents against rapidly growing aerobic bacteria involved in infection us diseases. Russian.
  • 6. ISO 20776-1:2006 “Clinical laboratory testing and in vitro diagnostic test systems – Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices – Part 1: Reference method for testing the in vitro activity of antimicrobial agents against rapidly growing aerobic bacteria involved in infectious diseases”.
  • 7. Torumkuney D., Papaparaskevas J., Morrissey I. Results from the Survey of Antibiotic Resistance (SOAR) 2014-16 in Greece. J Antimicrob Chemother. 2018;73(Suppl. 5):v36-v42. DOI: 10.1093/jac/dky068
  • 8. Torumkuney D., Nica M., Nistor I., Vatcheva-Dobrevska R., Petrovic V., Stoica A., et al. Results from the Survey of Antibiotic Resistance (SOAR) 2014-16 in Bulgaria, Romania, Serbia and Croatia. J Antimicrob Chemother. 2018;73(Suppl. 5):v2-v13. DOI: 10.1093/jac/dky066
  • 9. Torumkuney D., Pertseva T., Bratus E., Dziublik A., Yachnyk V., Liskova A., et al. Results from the Survey of Antibiotic Resistance (SOAR) 2014-16 in Ukraine and the Slovak Republic. J Antimicrob Chemother. 2018;73(Suppl. 5):v28-v35. DOI: 10.1093/jac/dky069
  • 10. Torumkuney D., Mayanskiy N., Edelstein M., Sidorenko S., Kozhevin R., Morrissey I. Results from the Survey of Antibiotic Resistance (SOAR) 2014-16 in Russia. J Antimicrob Chemother. 2018;73(Suppl. 5):v14-v21. DOI: 10.1093/jac/dky065
  • 11. Zhang Z, Chen M, Yu Y, Pan S, Liu Y. Antimicrobial susceptibility among Streptococcus pneumoniae and Haemophilus influenzae collected globally between 2015 and 2017 as part of the Tigecycline Evaluation and Surveillance Trial (TEST). Infect Drug Resist. 2019;12:1209-1220. DOI: 10.2147/IDR.S203121
  • 12. Sivaya O.V., Kozlov R.S., Krechikova O.I., Ivanchik N.V., Katosova L.K., Gudkova L.K., and PeGASus Study Group. Antimicrobial Resistance of Haemophilus influenzae in Russia: Results of Multicenter Study «PeGASus». Klinicheskaja mikrobiologija i antimikrobnaja himioterapija. 2014;16(1):5769. Russian.
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